Syndecan‐1 modulates leukocyte adhesion in the murine parietal peritoneum microcirculation in response to <i>Staphylococcus aureus</i> lipotechoic acid
Bibliographic record
Abstract
Syndecan‐1 modulates leukocyte recruitment in several tissues. The objective of this study was to determine the effects of syndecan‐1 blockade on leukocyte‐endothelial cell interactions in venules of the parietal peritoneum. BALB/c mice were stimulated with ip injection of E. coli LPS or S. aureus LTA and the microcirculation was examined by intravital microscopy after 4 hrs. Syndecan‐1 was localized to the basal membrane of venular endothelium using in vivo confocal imaging with a fluorescent anti‐syndecan‐1 antibody. Leukocyte‐endothelial interactions and leukocyte extravasation were not significantly different in response to LPS compared to naive control. After stimulation with LTA, rolling (94.8 ± 15.3 cells/min) and adhesion (15.0 ± 4.0 cells) along the endothelial wall and extravascular leukocytes (8.5 ±1.8 cells) were significantly increased compared to control, (37.0 ± 8.5, 1.26 ± 0.5, 0.7 ± 0.3 respectively, p < 0.05). Syndecan‐1 blockade before stimulation with LTA significantly increased the number of adherent leukocytes (37.8 ± 6.0, p < 0.001) and systemic neutrophil counts. Leukocyte‐endothelial interactions and systemic neutrophil counts were not increased in anti‐syndecan‐1‐injected saline controls. Our data suggest the parietal peritoneum vasculature is more responsive to LTA than LPS and syndecan‐1 is a negative regulator of leukocyte adhesion during LTA‐induced inflammation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".