Induction of experimental colitis by dextran sulphate sodium in a piglet model of irritable bowel syndrome
Bibliographic record
Abstract
This study was conducted for chemical induction of intestinal inflammation with dextran sodium sulphate (DSS) in piglets. A total of 11 Yorkshire piglets of 5 d of age, with an average body weight (BW) of 3 kg, were fitted with intra‐gastric catheters and divided into a control (CON, n=6) and treatment groups (DSS, n=5). Both groups were infused with equal volumes of either saline or 1.25 g of DSS/kg BW.d in saline, respectively, for 10 d. Histological grading of the colon indicated that DSS animals had severe, transmural inflammation. ELISA analysis indicated that DSS‐treated piglets had higher ( P <0.05) levels of the pro‐inflammatory cytokines TNF‐α and IL6 in the colon (DSS, 476.64±62.91 vs. CON, 83.66±22.52 and DSS, 189.72±9.93 vs. CON, 64.91±5.84, pg/g tissue), respectively. Quantitative real time RT‐PCR confirmed higher ( P <0.05) expression levels of TNF‐α mRNA in DSS‐treated pigs (DSS, 1.21±0.51 vs. CON, 0.011±0.002, arbitrary units) while the anti‐inflammatory cytokine IL10 was undetectable (DSS, ND vs. CON, 0.025±0.005, arbitrary units) in the colon of DSS‐treated piglets. Gut D‐mannitol permeability into blood circulation subsequent to an intra‐gastric bolus infusion of D‐mannitol (0.6 g/kg BW) after 75 min was higher ( P <0.05) in the DSS group (DSS, y = 1.691x, r 2 = 0.97 vs. CON, y = 0.463x, r 2 = 0.93). We conclude that the dosage of 1.25 g DSS/kg BW.d is suitable to induce experimental colitis and IBS pathology in piglets. Supported by OMAFRA of Canada.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.002 | 0.003 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".