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Record W846342693

Pathogenetic studies of sarcoma development in retriever breeds

2014· dissertation· en· W846342693 on OpenAlexaboutno aff
K.M. Boerkamp

Bibliographic record

VenueData Archiving and Networked Services (DANS) · 2014
Typedissertation
Languageen
FieldMedicine
TopicVeterinary Oncology Research
Canadian institutionsnot available
FundersEuropean Commission
KeywordsSarcomaAneuploidyPathologyCancerBiologyMesenchymal stem cellPopulationMedicineChromosomeGeneticsGene
DOInot available

Abstract

fetched live from OpenAlex

In the Dutch population of Golden retrievers, a predisposition for the development of cancer in general and certain types of cancer (such as mast cell tumours and possibly also soft tissue sarcomas (STS)) was shown to exist. In addition, age, location and incidence of various tumours differed as related to the diagnostical procedure of choice, being either cytology or histology, indicating a selection bias for diagnostic procedure. A subset of the collected cohort, consisting of unclassified sarcomas, (n=110) were selected for revision. Revision was based on morphological characteristics and, if deemed necessary, additional immunohistochemistry. In 58 tumours, we were able to arrive at the diagnosis of a specific STS. As many as 31 tumours (28%), however, were on revision not considered to be STS. Four neoplasms (4%) were considered non-neoplastic. These numbers illustrate the necessity for revision (and \\ possibly subclassification) of this group of tumours.
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\nDNA-aneuploidy may reflect a malignant nature of mesenchymal proliferations and herald gross genomic instability. Of all mesenchymal tumours examined within this study, half of all sarcomas were aneuploid. Benign mesenchymal neoplasms were rarely aneuploidy, and all of the inflammatory lesions were diploid. The determination of DNA ploidy status is considered to be of additional value when attempting to better differentiate between malignant and non-malignant mesenchymal lesions. In addition, hypoploidy appears more common in canine sarcomas and hyperploid cases show less deviation of the DNA index than human sarcomas. This strengthens the concept of interspecies variation in the evolution of gross chromosomal aberrations during cancer development.
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\nAiming to identify genes causing the suspected breed predispositionfor the development of STS in both the Labrador- and Golden retriever breed,a genome-wide association study was performed. Results were suggestive for a germ-line genetic connection between the development of STS in the Labrador- and Golden retriever breed. Within both breeds, different loci (all of which contained candidate genes already known to be involved in the development of cancer) could be identified.
\nThe histiocytic sarcoma is a specific sarcoma subtype that often develops in Flatcoated retrievers. Within this tumour, the significantly altered expression of nine genes and deregulation of gene signaling pathways were identified by means of a microarray study and additional q PCR confirmation. These genes were not yet implicated in histiocytic sarcoma manifestations in the dog nor in comparable human histiocytic/dendritic sarcomas.
\nHistiocytic sarcomas can be grouped mainly into a soft tissue- and visceral form. The Flatcoated retriever is predisposed mainly for the first subtype. Genetic differences possibly causing the phenotypical variation of these two histiocytic sarcoma subtypes were identified by microarray analyses. The significantly aberrant expression of three genes were confirmed by q PCR. These differences indicate that these two forms of histiocytic sarcoma in the dog display a variation in gene expression.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.350
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.064
GPT teacher head0.369
Teacher spread0.305 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

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