Glucocorticoid induces pentraxin 3 expression through mitogen-activated protein kinase pathway in human airway smooth muscle cells (P6013)
Bibliographic record
Abstract
Abstract Long pentraxin 3 (PTX3) is a multifunctional soluble pattern recognition receptor that links innate immunity, inflammation, and matrix deposition. Glucocorticoids (GC) are an important anti-inflammatory treatment in asthma. Previous studies have demonstrated that GC had divergent effects on PTX3 production in diverse cell types. In this study, we first evaluated whether GC alone induced and, under inflammatory conditions, enhanced and extended PTX3 production in Human airway smooth muscle cells (HASMC) and investigated the plausible molecule mechanism regulating the induction of PTX3. HASMC transfected with PTX3 promoter constructs were treated with dexamethasone (Dex) or and TNF. We demonstrated Dex induced and synergized with TNF to augment PTX3 protein levels. However, Dex does not influence the mRNA levels and promoter activity of PTX3 alone, but it decreased the effect of TNF-induced PTX3 promoter activity. Further results had shown that Dex increased TNF-induced PTX3 mRNA stability. Primary HASMC pretreated with inhibitors of p42/p44 ERK, but not p38 or JNK-MAPK, significant decrease in PTX3 release on Dex- and TNF-treatment. Taken together, our data provide the first evidence of Dex-induced PTX3 expression in HASMC maybe occurred posttranscriptional mechanisms and via p42/p44-MAPK signaling pathways. Our results raise the possibility that HASMC may play a role in airway inflammation via PTX3-dependent pathway.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".