Determination of VraR Binding Activity on fmtA Promoter
Bibliographic record
Abstract
In S. aureus , the VraSR two‐component system can sense cell wall stress and coordinate the response to antibiotics that inhibit cell wall peptidoglycan biosynthesis. fmtA encodes a low‐affinity penicillin binding protein and is part of the core cell wall stimulon. fmtA is described to be under the regulation of VraSR. In this study, we investigated binding of VraR to the fmtA promoter region. Two VraR binding regions in fmtA promoter have been found by EMSA and DNase I footprinting assays. The VraR binding motifs on these regions were predicted, and single or double nucleotide mutations were generated to investigate, the recognition or binding elements for VraR. We are using runoff in vitro transcription assays to investigate further the binding mode of VraR. The qRT‐PCR showed that the fmtA expression level was upregulated by VraS/VraR under oxacillin treatment. Our study suggests that VraR coordinates fmtA expression in vivo . Source of research support: CIHR Canadian Institutes of Health Research.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".