Regulation of the CDP‐ethanolamine (Kennedy) pathway by oxysterols
Bibliographic record
Abstract
The CDP‐ethanolamine (Kennedy) pathway synthesizes phosphatidylethanolamine (PE) and PE plasmalogens de novo from ethanolamine and diacylglycerols or alkylacyl glycerols, respectively. The intermediate reaction in which CDP: ethanolaminephosphate cytidylyltransferase (Pcyt2) catalyzes the formation of CDP‐ethanolamine from CTP and ethanolaminephosphate is the main regulatory step in this pathway. Pulse‐chase radiolabeling experiments of the mouse embryonic cells C3H10T1/2 with 14C‐ethanolamine demonstrate that 14C‐CDP‐ethanolamine synthesis decreases 70% after 48h treatments with 25‐hydroxycholesterol. After radiolabeling with 14C‐ethanolamine and 3H‐glycerol PE synthesis decreases 2‐fold under the same conditions. The regulation is at the step catalyzed by Pcyt2. Analysis of the mouse Pcyt2 promoter demonstrates that two regions could be responsive to the oxysterol treatments. The promoter region between −475bp and ‐302 bp contains the consensus binding sites for Ap1, MyoD, cEBP transcription factors; the region located within the −252/−185 bp area contains bonding sites for Sp1.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".