Synthetic Genetic Array (SGA) analysis of sec14 cki1 identifies a downstream role for the Golgi specific TRAPPII‐Rab/Ypt31 signaling cascade.
Bibliographic record
Abstract
The yeast phosphatidylcholine/phosphatidylinositol transfer protein Sec14p is indispensable for vesicular transport from the Golgi and cell viability. The essential nature of Sec14p function can be bypassed by inactivation of other genes. These include enzymes in the CDP‐choline pathway for phosphatidylcholine biosynthesis and the yeast oxysterol binding protein homologue Kes1p/Osh4p. To further understand the mechanism of sec14 bypass we performed a series of synthetic lethal analyses with each of approximately 4800 single deletion mutants. The results indicate ~ 31 genes required for the viability of the sec14 cki1 strain. Included within the 31 genes were components of the TRAPPII complex and ypt31 but not the closely related ypt32 . In addition, these genes are not required for the viability of the sec14 kes1 strain, demonstrating a specific requirement for this mode of bypass. Furthermore, inactivation of the KES1 gene ameliorated the growth defects associated with the sec14 cki1 synthetic lethal interactions with the TRAPPII components and ypt31 . Together the results demonstrate the differences between the modes of sec14 bypass. As well the results suggest that Kes1p may function as an inhibitor of Ypt31p, the TRAPPII complex, or possibly a protein upstream of TRAPPII function. (Supported by the Canadian Institutes of Health Research and the Canada Research Chairs fund)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".