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Record W958342678 · doi:10.1128/9781555815660.ch74

<i>Birc1e/Naip5</i>in Macrophage Function and Susceptibility to Infection with<i>Legionella pneumophila</i>

2014· book-chapter· en· W958342678 on OpenAlex
A H Fortier, Philippe Gros

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueASM Press eBooks · 2014
Typebook-chapter
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicLegionella and Acanthamoeba research
Canadian institutionsMcGill University
Fundersnot available
KeywordsLegionella pneumophilaPhagosomeMicrobiologyBiologyMacrophageLegionellaEndoplasmic reticulumIntracellularIntracellular parasiteImmune systemCell biologyPhagocytosisBacteriaImmunologyIn vitroGenetics

Abstract

fetched live from OpenAlex

Genetic analysis in the mouse has been used to identify host genes and proteins that play important roles in natural defences against a broad range of infectious diseases. In mouse, susceptibility to infection with Legionella pneumophila is genetically controlled. Intracellular pathogens have evolved different strategies to inhibit or escape the bacteriostatic or bactericidal defense mechanisms of host macrophages. Electron microscopy studies indicated that Legionella-containing phagosome (LCP) morphology diverges from classic phagosomes: within the first 5 min of infection, LCPs become surrounded by host vesicles; 15 min postinfection, the thickness of the phagosomal membrane resembles that of the endoplasmic reticulum (ER), and 6 h after infection, ribosomes are found attached to the cytoplasmic face of LCPs. Nontransgenic permissive A/J peritoneal and bone marrow-derived macrophages allowed massive L. pneumophila replication over a 72-h infection period (2.8 ± 0.3 and 1.6 ± 0.4 log CFU respectively) compared to macrophages from Birc1e/Naip5-resistant transgenic animals (0.74 ± 0.18 and -0.52 ± 0.26 log CFU respectively). It has been proposed that, like other members of the NLR family, Birc1e/Naip5 may function as an intracellular sensor of L. pneumophila products and may trigger immune responses through inflammatory caspases.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Other · Consensus signal: none
Teacher disagreement score0.934
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.237
Teacher spread0.222 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it