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Cytokine priming enables triggering of autoreactive CD8 T lymphocytes by low affinity TCR ligands (83.7)

2010· article· en· W99386962 on OpenAlexaff
Subburaj Ilangumaran, Stéphanie Dubois, Xi Lin Chen, Chantal LeBlanc, Pamela S. Ohashi, Sheela Ramanathan

Bibliographic record

VenueThe Journal of Immunology · 2010
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicDiabetes and associated disorders
Canadian institutionsOntario Institute for Cancer ResearchCentre Hospitalier Universitaire de SherbrookeUniversité de Sherbrooke
Fundersnot available
KeywordsPriming (agriculture)ImmunologyCytotoxic T cellT-cell receptorBiologyCytokineCD8Cell biologyInterleukin 21AntigenT cellImmune systemIn vitroBiochemistry

Abstract

fetched live from OpenAlex

Abstract The cellular and molecular events preceding the initial activation of autoreactive CD8 T cells in several autoimmune diseases remain unclear. Recent reports have implicated IL-6, IL-21, IL-15 and IL-7 in the triggering of autoreactive CD8 T cells in mouse models of autoimmune diseases. We have shown that IL-21 and IL-6 synergize with IL-7 or IL-15 to induce antigen-independent proliferation of naive CD8 T cells, with a concomitant increase in antigen responsiveness. We postulated that the cytokine-induced augmentation of TCR sensitivity (referred herein as ‘cytokine priming’) might enable stimulation of potentially autoreactive CD8 T cells by low affinity self antigens. Here, we show that cytokine priming enables naive TCR transgenic CD8 T cells to respond to low affinity TCR ligands, resulting in proliferation and acquisition of effector functions. We have used a transgenic mouse model of autoimmune type 1 diabetes to show that cytokine-primed autoreactive CD8 T cells acquire the capacity to cause disease following stimulation with weak TCR agonists, and that the diabetogenic potential of these cells is dependent on the continuous availability of IL-15. These findings demonstrate a novel mechanism by which cytokines contribute to the triggering of autoreactive CD8 T cells and have important implications for autoimmune diseases associated with infections and chronic inflammation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.004
GPT teacher head0.207
Teacher spread0.204 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2010
Admission routes1
Has abstractyes

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