Influencing Fatty Acids Composition of Yeasts by Lanthanides
Notice bibliographique
Résumé
The growth of microorganisms is affected by cultivation conditions, concentration of carbon and nitrogen sources and the presence of trace elements. One of new possibilities of influencing the production of biomass or lipids is the use of lanthanides. Lanthanides are biologically non-essential elements with wide applications in technology and industry and their concentration as environmental contaminants is therefore increasing. Although non-essential, lanthanides have been proposed (and even used) to produce beneficial effects in plants but their mechanisms of action are unclear. Recently, it was suggested that they may replace essential elements. We tested the effect of low concentrations of lanthanides on traditional biotechnologically useful yeast species (Kluyveromyces polysporus, Saccharomyces cerevisiae, Torulospora delbrueckii), and species capable of high accumulation of lipids (Rhodotorula glutinis, Trichosporon cutaneum, Candida sp., Yarrowia lipolytica). Growth characteristics were determined in the above yeast strains cultivated with different lanthanides or monazite (calculated to lanthanum proportional content) concentrations (0.03; 0.3; 1; 3 mg/L). The cultivations were carried out for 96 h (until early stationary phase), cultivation temperature was 30 C. The fatty acids extraction was based on the method of Bligh and Dyer (1959). Gas chromatography-mass spectrometry of fatty acid picolinyl esters was done on a GC-MS system.The lanthanides in selected concentration did not have any significant inhibitory effect on yeast growth and some of them even showed a stimulatory effect. Low concentrations of some lanthanides were conducive to an increase in biomas and also higher production of palmitoleic acid, commonly used in cosmetics and medicine, and 6-linoleic acid which is a precursor of thromboxanes, prostaglandins and leucotrienes. Lanthanum enhanced the cell biomass of T. cutaneum by as much as 150% and other individual selected lanthanides (lanthanum, praseodymium, gadolinium and monazite as a naturally occurring mixture of lanthanides) in the range of 23 and 57 %. Cultivation with monazite increased the cell biomass in Candida sp., R. glutinis and T. cutaneum by 30 -40% and in Y. lipolytica by 60%. A sizable increase in lipid content occurred upon addition of praseodymium and lanthanum to K. polysporus (from 5 % to 9 %), Candida sp. (from 35 % to 43 %), Y. lipolytica (from 18 % to 25 %) and a 10-fold higher concentration of monazite (3 mg/l) boosted the increase of lipid content by some 50% compared to the effect of 0.3 mg/l. The highest increase of the palmitoleic and linoleic acid content was brought about by lanthanum, and in some strains by monazite irrespective of its concentration. The highest yield of palmitoleic and linoleic acid, in excess of 300 mg/g, was obtained by culturing T. cutaneum with lanthanum and monazite.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».