Bibliographic record
Abstract
HCMV encodes a plethora of immune-modulating functions, many of which have yet to \nbe assigned to specific genes. In prospect of performing high-throughput screens to \nidentify and characterise such functions, a library of recombinant adenoviruses (RAds) \neach encoding a V5 epitope-tagged HCMV protein was generated. Protein expression \nwas validated and characterised for the vast majority of RAds by western blot and \nimmunofluorescence. \nHCMV has been reported to both upregulate cell surface expression of Fas, and render \ncells resistant to Fas-mediated killing. This thesis demonstrated that Fas levels are \nmarkedly reduced at the surface of HCMV-infected cells as an early function that \npersists through the late phase. Screening a panel of HCMV deletion mutants \neliminated 83 genes as not required for Fas downregulation, while screening the RAd \nlibrary did not identify any single HCMV gene as being sufficient for this function. \nDeep sequencing of the HCMV transcriptome recently led to the identification of \nUL150A as a novel protein-coding gene. To test this prediction, UL150A was tagged \nwithin the strain Merlin genome. UL150A was shown to encode multiple protein \nproducts, and be expressed with early and late kinetics. \nIn a screen of the RAd library, gpUL4 was observed to be secreted from cells. To \ninvestigate this function in the context of HCMV infection, an epitope-tag was inserted \nat the 3’-end of the UL4 gene in the strain Merlin genome. Tagged gpUL4 was secreted \nfrom cells infected with strain Merlin. Secreted gpUL4 was more heavily glycosylated, \nand produced in greater abundance than its intracellular counterpart late in infection. \nActive secretion would be consistent with gpUL4 acting as a virokine, cytokine or \ncytokine/chemokine-binding protein. gpUL4 purified from supernatants of Merlin- or \nRAd-UL4-infected cells inhibited NK cell degranulation. Furthermore, gpUL4 did not copurify \nwith virus particles, indicating it is unlikely to be a virion component.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".