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Record W1508974656 · doi:10.1002/9780470027318.a0107

Infrared Spectroscopy, Ex Vivo Tissue Analysis by

2000· other· en· W1508974656 on OpenAlex
Michael Jackson, Henry H. Mantsch

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueEncyclopedia of Analytical Chemistry · 2000
Typeother
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSpectroscopy Techniques in Biomedical and Chemical Research
Canadian institutionsNational Research Council Institute for Biodiagnostics
Fundersnot available
KeywordsNucleic acidRaman spectroscopyInfrared spectroscopySpectroscopyIntramolecular forceChemistryInfraredCovalent bondBiological systemAnalytical Chemistry (journal)Nuclear magnetic resonanceBiophysicsBiochemistryBiologyOpticsPhysicsStereochemistryOrganic chemistry

Abstract

fetched live from OpenAlex

Abstract Infrared (IR) spectroscopy provides information relating to the vibration of covalent bonds within molecules. The wavelength of light absorbed by a vibrating bond depends upon the atoms in the bond, the type of bond, the type of vibration and inter‐ and intramolecular interactions. For complex samples such as human tissues an IR spectrum therefore provides a direct indication of sample biochemistry. With the correct choice of sampling methodology (usually an IR microscope) information on the biochemical nature of disease states can be obtained from tissue samples, which can often be useful diagnostically. Variations in spectral signatures arising from nucleic acids, proteins and lipids can provide important information in a number of disease states, including Alzheimer's disease (AD), breast cancer and skin cancer. The information obtained by IR spectroscopy is difficult to obtain with many other instrumental techniques. For example the signal‐to‐noise ratio obtained by IR microscopy is far superior to that seen with Raman methods, allowing more sophisticated data processing and so more information to be extracted. Furthermore, many species of interest cannot be studied in situ with other techniques. Nucleic acids are a case in point. Powerful techniques such as nuclear magnetic resonance spectroscopy provide no information concerning this material, while Raman techniques only provide information relating to individual nucleotides. In contrast, DNA and RNA give rise to IR signatures that provide information relating to nucleic acid content/structure. To appreciate fully the information contained in the complex spectra obtained from human tissues and cells, a unique combination of expertise in spectroscopy, biochemistry and anatomy/histology is required. This combination allows the investigator to avoid potential artefacts due to incorrect sampling and spatial variations in sample composition and to attribute the major absorptions present in spectra to individual biochemical species. However, spectral interpretation is often a highly subjective process, a fact that is made worse when one considers that many thousands of spectra are often acquired from a single tissue section. The application of pattern recognition techniques to IR data removes this subjectivity and allows realistic processing of these large data sets. In addition, many new methods are being developed which allow presentation of these complex data sets in a form readily interpreted by the nonexpert.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Insufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.223
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0340.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.004
GPT teacher head0.288
Teacher spread0.284 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it