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Record W1908842189 · doi:10.1002/9780470027318.a0206

Fluorescence‐Based Biosensors

2000· other· en· W1908842189 on OpenAlex
John D. Brennan

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueEncyclopedia of Analytical Chemistry · 2000
Typeother
Languageen
FieldEngineering
TopicBiosensors and Analytical Detection
Canadian institutionsMcMaster University
Fundersnot available
KeywordsBiosensorAnalyteBiomoleculeNanotechnologyTransducerFluorescenceBioelectronicsChemistryMaterials scienceChromatography

Abstract

fetched live from OpenAlex

Abstract Biosensors, as defined by Biosensors and Bioelectronics, are analytical devices “incorporating a biological material, a biologically derived material or biomimetic intimately associated with or integrated within a physicochemical transducer or transducing microsystem”. Fluorescence‐based biosensors are those devices that derive an analytical signal from the fluorescence emission process. Such biosensors may be used for a wide variety of tasks, including: detection of compounds of biomedical, 1 environmental 2 or defense interest, 3 on‐line monitoring for process control, 4 monitoring of foodstuffs, 5 selective detection of compounds undergoing a chemical separation, 6 and screening of drug compounds. 7 Advantages of such devices include: 8 high selectivity; rapid response times; reusability; amenability to remote analysis; and immunity from electrical interferences. The selective nature of the complexation between the biomolecule and the analyte, combined with the small size of the device and the advantages of total internal reflection (TIR)‐based spectroscopy, 9 also results in an ability to measure analytes in complex matrixes. Such samples may include highly scattering systems such as milk or whole blood, 10 or relatively inaccessible locations such as groundwater wells, or even intracellular environments. 11 The key limitation of such devices mainly centers around the poor stability of biological compounds, which can lead to a substantial drift in instrument response over time. There is also the potential for interferences related to biological autofluorescence, and the analyte‐dependent sensitivity and limit of detection (LOD) for sensors, which rely on the nature of both the protein and the fluorescent probe utilized. Finally, in cases where immunological reagents are used, the devices can show a lack of reversibility, and may operate only as a “one‐shot” screen, without the potential for continuous, quantitative analysis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Insufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.031
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0120.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.208
Teacher spread0.202 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it