Monitoring in Real-Time the Degrafting of Covalently Attached Fluorescent Polymer Brushes Grafted to Silica Substrates—Effects of pH and Salt
Bibliographic record
Abstract
Poly(acrylic acid) (PAA) covalently immobilized on glass substrates was made fluorescent by grafting a BODIPY derivative (PMOH) via an ester linkage. Although only nanograms/square centimeter of polymer are understood to be immobilized onto the SiO 2 substrate, the fluorophore-tagged polymer was readily visible to the naked eye and its fluorescence was easily detected. The characteristic BODIPY emission, centered at 550 nm, was used to follow the degrafting of PAA from the glass substrates in aqueous solution in real-time using total internal reflection fluorescence (TIRF) microscopy. The substrate−initiator bond hydrolysis and the conditions at which the PAA degrafting occurred were unequivocally confirmed in real-time by TIRF microscopy. No cleavage of the polymer occurred between pH 6.5 and 10.5 in the absence of NaCl. In contrast, polymer degrafting from the substrate occurred at pH ≥ 9.5 when 10 mM NaCl was added to the buffer solution.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".