Methyl Oleate Production in a Supported Sol–Gel Immobilized Lipase Packed Bed Reactor
Bibliographic record
Abstract
A supported immobilized lipase was investigated as a suitable biocatalyst for the transesterification of triolein to methyl oleate in a packed bed reactor (PBR). The packing material for the bioreactor was comprised of lipase immobilized in sol–gel and supported on Celite (CSG). A kinetic- and mass transfer-based model was developed, incorporating a novel efficiency correlation to account for the effect of glycerol on the immobilized enzyme. Comparing the CSG PBR performance to that using a commercial lipase preparation (Novozym 435 (N435)), higher product conversion was achievable using CSG over a shorter time period, indicating the superior performance of CSG for enzymatic transesterification. A rate equation accounting for alcohol inhibition was used to compare the kinetics of the CSG and N435 PBRs. It was found that CSG exhibited a higher maximum reaction rate and was less prone to inhibition by methanol relative to N435. The resulting model showed good agreement with the experimental data, and CSG exhibited good stability without loss of activity over a five day period. This biocatalyst shows significant potential for the efficient production of biodiesel, and the mechanistic model developed has broader applications for similar enzyme and reaction systems.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".