Transcript expression profile of water-limited roots of hexaploid wheat (<i>Triticum aestivum</i>‘Opata’)
Bibliographic record
Abstract
Triticum aestivum 'Opata' is an elite hard red spring wheat that has been used as a parent of the ITMI (International Triticeae Mapping Inititative) mapping population and also in the production of synthetically derived hexaploid wheats, some of which (following selection) show increased drought tolerance relative to Opata. Here, we describe the response of Opata roots to water withholding, using physiological variables and oligonucleotide microarrays. We identified 394 distinct transcripts whose abundance differed (p < or = 0.05) at least 1.5-fold between water-limited and control roots of Opata, of which 190 transcripts increased and 204 decreased following water limitation. In addition to previously characterized markers of abiotic stress and many genes of unknown function, we identified multiple putative glucanases and class III peroxidases as being particularly responsive to stress. We also compared these data to previously described microarray analyses of Opata's more drought-tolerant, synthetic-derived progeny, and found a relatively high correlation (r = 0.7) between responsive transcripts in the two genotypes, despite differing physiological responses. Some of the transcripts that we confirmed by qRT-PCR as being differentially expressed between Opata and the more tolerant synthetic-derived genotype under stress include a class III peroxidase, an AP2-family transcription factor, and several transcripts of unknown function.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".