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Record W2085042277 · doi:10.1038/ncomms7351

Epigenetic and transcriptional determinants of the human breast

2015· article· en· W2085042277 on OpenAlexafffund
Philippe Gascard, Misha Bilenky, Mahvash Sigaroudinia, Jianxin Zhao, Luolan Li, Annaïck Carles, Allen Delaney, Angela Tam, Baljit Kamoh, Stephanie Cho, Malachi Griffith, Andy Chu, Gordon Robertson, Dorothy Cheung, Irene Li, Alireza Heravi‐Moussavi, Michelle Moksa, Matthew Mingay, Angela Hussainkhel, Brad Davis, Raman P. Nagarajan, Chibo Hong, Lorigail Echipare, Henriette O’Geen, Matthew J. Hangauer, Jeffrey B. Cheng, Dana S. Neel, Donglei Hu, Michael T. McManus, Richard A. Moore, Andrew J. Mungall, Yussanne Ma, Patrick Plettner, Elad Ziv, Ting Wang, Peggy Farnham, Steven J.M. Jones, Marco A. Marra, Thea D. Tlsty, J Costello, Martin Hirst

Bibliographic record

VenueNature Communications · 2015
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEpigenetics and DNA Methylation
Canadian institutionsUniversity of British ColumbiaCanada's Michael Smith Genome Sciences CentreBC Cancer Agency
FundersNational Institute on Drug AbuseNational Human Genome Research InstituteKaiser Foundation Research InstituteNational Institutes of HealthGenome British ColumbiaNational Institute of Environmental Health SciencesCanadian Institutes of Health ResearchCanada's Michael Smith Genome Sciences CentreNatural Sciences and Engineering Research Council of CanadaJohns Hopkins University
KeywordsEpigenomicsEpigeneticsMyoepithelial cellBiologyReprogrammingDNA methylationCell typeGeneticsEpigenesisTiling arrayCellular differentiationEnhancerComputational biologyCellTranscriptomeGeneGene expressionImmunology

Abstract

fetched live from OpenAlex

While significant effort has been dedicated to the characterization of epigenetic changes associated with prenatal differentiation, relatively little is known about the epigenetic changes that accompany post-natal differentiation where fully functional differentiated cell types with limited lifespans arise. Here we sought to address this gap by generating epigenomic and transcriptional profiles from primary human breast cell types isolated from disease-free human subjects. From these data we define a comprehensive human breast transcriptional network, including a set of myoepithelial- and luminal epithelial-specific intronic retention events. Intersection of epigenetic states with RNA expression from distinct breast epithelium lineages demonstrates that mCpG provides a stable record of exonic and intronic usage, whereas H3K36me3 is dynamic. We find a striking asymmetry in epigenomic reprogramming between luminal and myoepithelial cell types, with the genomes of luminal cells harbouring more than twice the number of hypomethylated enhancer elements compared with myoepithelial cells.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.031
GPT teacher head0.320
Teacher spread0.289 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations81
Published2015
Admission routes2
Has abstractyes

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Same venueNature CommunicationsSame topicEpigenetics and DNA MethylationFrench-language works237,207