Genomic analysis identifies association of <i>Fusobacterium</i> with colorectal carcinoma
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
No Canadian affiliation. An affiliation-only frame — the usual design — would never have seen this work. It is one of the works that make the case for inverting the frame.
Abstract
The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- Genome Research
- Topic
- Gut microbiota and health
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- —
- Funders
- National Center for Advancing Translational SciencesNational Institute of Diabetes and Digestive and Kidney DiseasesNational Cancer InstituteNational Human Genome Research InstituteUniversity of GuelphNatural Sciences and Engineering Research Council of CanadaBroad InstituteAlbert J. Ryan FoundationDamon Runyon Cancer Research Foundation
- Keywords
- FusobacteriaBiologyColorectal cancerFusobacteriumCarcinomaCarcinogenesisFirmicutesCancerGeneticsGeneBacteroides16S ribosomal RNABacteria
- Has abstract in OpenAlex
- yes