Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Abstract
mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the alpha and beta diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.
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The record
- Venue
- Applied and Environmental Microbiology
- Topic
- Microbial Community Ecology and Physiology
- Field
- Environmental Science
- Canadian institutions
- Dalhousie UniversityUniversity of British Columbia
- Funders
- Alfred P. Sloan FoundationNational Science Foundation
- Keywords
- LaptopSoftwarePyrosequencingComputer scienceMetagenomicsData miningComputational biologyBiologySoftware engineeringGeneticsGeneProgramming languageOperating system
- Has abstract in OpenAlex
- yes