Crystallographic and Biophysical Analysis of a Bacterial Signal Peptidase in Complex with a Lipopeptide-based Inhibitor
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
We report here the crystallographic and biophysical analysis of a soluble, catalytically active fragment of the Escherichia coli type I signal peptidase (SPase Δ2–75) in complex with arylomycin A2. the with of the in the of the and and type with the of the and the of in the the complex and the the of signal peptidase and in the of We report here the crystallographic and biophysical analysis of a soluble, catalytically active fragment of the Escherichia coli type I signal peptidase (SPase Δ2–75) in complex with arylomycin A2. the with of the in the of the and and type with the of the and the of in the the complex and the the of signal peptidase and in the of I signal peptidase signal the of coli signal peptidase the and the signal the of coli signal peptidase the and the the the of the signal and the and the Escherichia coli the and the and and coli and a and a catalytically active fragment of (SPase Δ2–75) the the and the and the and of in complex with a in the of coli the of signal peptidase a of and of of I the and of the of with a and the of the I and in signal and crystallographic with a the and the analysis of the coli a and a the the with and the and the and the in the of the the the in and in type I signal peptidase and in coli the of coli with the the coli signal with the a of a of of in in with a the the the of the in the of the a a the of the and crystallographic and biophysical analysis of the of of in the of coli signal peptidase the of and the in and type I signal analysis of and in and arylomycin and in and the a and and with a of and of of the and of the the with of a the and the the in the the in a of the the with the of the and of I the of and the of I and a of of the and and the and in the the in and in a and the the of coli a and analysis with the the of the the and the the and of the with the analysis with the and the the the in the the the and the and the the and the of in the the the and and the and arylomycin in the active of signal the signal peptidase arylomycin of the active of signal peptidase with a of arylomycin in the active of coli type I in and the in with and of the active in the and of signal the active in and the arylomycin signal peptidase active in the active in and the arylomycin signal peptidase active in the complex with the the complex and the of a and and and and and in and in and of of arylomycin and a in a and the a with a the the arylomycin the of the the the the analysis the of the and of of with the and the in the and of with the and the in the and in and a of the the in and the with and and and the of of the and the of the the and the of of signal peptidase a of in with and arylomycin and the in with of the a of and a and the of the and of the of with arylomycin a in a of and of in the the the of the the and the in of the of coli I the arylomycin of the with the here the crystallographic and the in and the complex the and the the arylomycin complex here in and the the with the the the the in the of the of in with the the the in the a with a with the of the of the with the and the and analysis of arylomycin of and of the and and a with a the of in the of of coli arylomycin in the active with the active in a with of the the of and of the of the and of the and in the with of the and in the of the with and the and in and in the of arylomycin the active with of the the the and the the the the of the a the and the the the the the the the in the in the of the in and in the in the of the in a with the and of with the of the and the of the in the of the and the the complex the of the in the in with the and with a the the the the of a with the of the of the active a of the the of arylomycin and the of the of in the the and the of the in the the in a with the in the in the arylomycin in the arylomycin the the of with arylomycin with the arylomycin of the in the of arylomycin peptidase complex a of the complex of the the of the in a in the analysis of the the of the of the of the the and a in and in in the arylomycin complex a in a of in the with the of a the of with the in the analysis the the the and of the in the a of arylomycin A2. the and the arylomycin the the complex the of the with and the and the of of arylomycin in the active of a in the of the of in the active of the the arylomycin of the complex the of the a of the and a in the of the arylomycin in with the of arylomycin a of the of a a of the the of the the of the of and the the the of of the a a the of arylomycin a and of arylomycin signal peptidase of a of of arylomycin a the a a a the a a in a of a of of arylomycin a the a a the a in a of arylomycin signal peptidase of and the in a and the of in of the of the of the of the in the in the of the a of the of the in and the of the of the a the the in the of and the with the a a of the and a of the the of the We a the and the arylomycin the with the arylomycin and the active and and the the complex the of of and the of the and the of the arylomycin a the of of and a the of the of the in of the and of the the the of the the of the of of with and the of the the a the a the in a in the arylomycin in of in the with the of and the of and the and the of signal of signal peptidase and signal peptidase with arylomycin in with a and a of of of arylomycin of a of and a of the and the the a the of the the and the the of and in in of the the of the and of signal of signal peptidase in the in of of arylomycin the the and of the signal peptidase arylomycin A2. a of a of in the type of the of arylomycin and arylomycin the arylomycin in the in the of the a arylomycin in the and a of the with the the of the arylomycin a of the of in a and the in arylomycin the of the in and a arylomycin the of and in complex with the a of the of a in the in the in arylomycin the and the of arylomycin the with signal with the arylomycin the analysis of arylomycin the the the and the signal peptidase the of arylomycin the of the the of the the the in in the in in signal peptidase with of the in the of the the of arylomycin of the and the crystallographic analysis and the with arylomycin a with a a a the and the a the of the of the active of the and the complex with arylomycin the of the of the of the in the active of a in the of the a of a in the of and analysis of the and of arylomycin in the of in the arylomycin the in the active of the of in the the and of in with the of the biophysical with the crystallographic analysis the arylomycin of the the of the and arylomycin and the of the complex the of and the of in the in the the and of the a the and the of the and with the of a signal in the active of coli signal peptidase the of the the and the complex with the and of the of the the of the the with the the the of arylomycin in the the of arylomycin a the the with the the the the the the analysis of signal and the of the the and the the of the signal a and with of with and the I signal peptidase signal the of coli signal peptidase the and the signal the of coli signal peptidase the and the the the of the signal and the and the Escherichia coli the and the and and coli and a and a catalytically active fragment of (SPase Δ2–75) the the and the and the and of in complex with a in the of coli the of signal peptidase a of and of of I the and of the of with a and the of the I and in signal and crystallographic with a the and the analysis of the coli a and a the the with and the and the and the in the of the the the in and in type I signal peptidase and in coli the of coli with the the coli signal with the a of a of of in in with a the the the of the in the of the a a the of the and crystallographic and biophysical analysis of the of of in the of coli signal peptidase the of and the in and type I signal analysis of and in and arylomycin and in and the a and and with a of and of of the and of the the with of a the and the the in the the in a of the the with the of the and of I the of and the of I and a of of the and and the and in the the in and in a and the the of coli a and analysis with the the of the the and the the and of the with the analysis with the and the the the in the the the and the and the the and the of in the the the and and the and of the active of signal peptidase with a of arylomycin in the active of coli type I in and the in with and of the active in the and of signal the active in and the arylomycin signal peptidase active in the active in and the arylomycin signal peptidase active in the complex with the the complex and the of a and and and and and in and in and of of arylomycin and a in a and the a with a the the arylomycin the of the the the the analysis the of the and of of with the and the in the and of with the and the in the and in and a of the the in and the with and and and the of of the and the of the the and the of of signal peptidase a of in with and arylomycin and the in with of the a of and a and the of the and of the of with arylomycin a in a of and of in the the the of the the and the in analysis of and in and arylomycin and in and the a and and with a of and of of the and of the the with of a the and the the in the the in a of the the with the of the and of I and the the of coli a and analysis with the the of the the and the the and of the with the analysis with the and the the the in the the the and the and the the and the of in the the the and and the and the complex with the the complex and the of a and and and and and in and in and of of arylomycin and a in a and the a with a the the arylomycin the of the the the the analysis the of the and of of with the and the in the and of with the and the in the and in and a of the the in and the with and and and the of of the and the of the the and the of of signal peptidase a of in with and arylomycin and the in with of the a of and a and the of the and of the of with arylomycin a in a of and of in the the the of the the and the in of the of coli I the arylomycin of the with the here the crystallographic and the in and the complex the and the the arylomycin complex here in and the the with the the the the in the of the of in with the the the in the a with a with the of the of the with the and the and analysis of arylomycin of and of the and and a with a the of in the of of coli arylomycin in the active with the active in a with of the the of and of the of the and of the and in the with of the and in the of the with and the and in and in the of arylomycin the active with of the the the and the the the the of the a the and the the the the the the the in the in the of the in and in the in the of the in a with the and of with the of the and the of the in the of the and the the complex the of the in the in with the and with a the the the the of a with the of the of the active a of the the of arylomycin and the of the of in the the and the of the in the the in a with the in the in the arylomycin in the arylomycin the the of with arylomycin with the arylomycin of the in the of arylomycin peptidase complex a of the complex of the the of the in a in the analysis of the the of the of the of the the and a in and in in the arylomycin complex a in a of in the with the of a the of with the in the analysis the the the and of the in the a of arylomycin A2. the and the arylomycin the the complex the of the with and the and the of of arylomycin in the active of a in the of the of in the active of the the arylomycin of the complex the of the a of the and a in the of the arylomycin in with the of arylomycin a of the of a a of the the of the the of the of and the the the of of the a a the of arylomycin a and of a of of arylomycin a the a a a the a a in a of a of of arylomycin a the a a the a in a of arylomycin signal peptidase of and the in a and the of in of the of the of the of the in the in the of the a of the of the in and the of the of the a the the in the of and the with the a a of the and a of the the of the We a the and the arylomycin the with the arylomycin and the active and and the the complex the of of and the of the and the of the arylomycin a the of of and a the of the of the in of the and of the the the of the the of the of of with and the of the the a the a the in a in the arylomycin in of in the with the of and the of and the and the of signal of signal peptidase and signal peptidase with arylomycin in with a and a of of of arylomycin of a of and a of the and the the a the of the the and the the of and in in of the the of the and of signal of signal peptidase in the in of of arylomycin the of the of coli I the arylomycin of the with the here the crystallographic and the in and the complex the and the the arylomycin complex here in and the the with the the the the in the of the the arylomycin of the with the here the crystallographic and the in and the complex the and the the arylomycin complex here in and the the with the the the the in the of the of in with the the the in the a with a with the of the of the with the and the and analysis of arylomycin of and of the and and a with a the the the the in the a with a with the of the of the with the and the and analysis of arylomycin of and of the and and a with a the of in the of of coli arylomycin in the active with the active in a with of the the of and of the of the and of the and in the with of the and in the of the with and the and in and in the of arylomycin the active with of the the the and the the the the of the a the and the the the the the the the in the in the of the in and in the in the of the in a of of coli arylomycin in the active with the active in a with of the the of and of the of the and of the and in the with of the and in the of the with and the and in and in the of arylomycin the active with of the the the and the the the the of the a the and the the the the the the the with the and of with the of the and the of the in the of the and the the complex the of the in the in with the and with a the the the the of a with the of the of the active a of the the of arylomycin and the of the of in the the and the of the in the the in a with the in the in the arylomycin in the arylomycin the the of with arylomycin with the arylomycin of the in the of arylomycin peptidase complex a of the complex of the the of the in a in the analysis of the the of the of the of the the and a in and in in the arylomycin complex a in a of in the with the of a the of with the in the analysis the the the and of the in the a of arylomycin A2. the and the arylomycin the the complex the of the with and the and with the of the and the of the in the of the and the the complex the of the in the in with the and with a the the the the of a with the of the of the active a of the the of arylomycin and the of the of in the the and the of the in the the in a with the in the in the arylomycin in the arylomycin the the of with arylomycin with the arylomycin of the in the of arylomycin peptidase complex a of the complex of the the of the in a in the analysis of the the of the of the of the the and a in and in in the arylomycin complex a in a of in the with the of a the of with the in the analysis the the the and of the in the a of arylomycin A2. the and the arylomycin the the complex the of the with and the and the of of arylomycin in the active of a in the of the of in the active of the the arylomycin of the complex the of the a of the and a in the of the arylomycin in with the of arylomycin a of the of a a of the the of the the of the of and the the the of of the a a the of arylomycin a and of a of of arylomycin a the a a a the a a in a of a of of arylomycin a the a a the a in a of arylomycin signal peptidase of and the in a and the of in of the of the of the of the in the in the of the a of the of the in and the of the of the a the the in the of and the with the a a of the and a of the the of the We a the and the arylomycin the with the arylomycin and the active and and the the complex the of of and the of the and the of the arylomycin a the of of and a the of the of the in of the and of the the the of the the of arylomycin in the active of a in the of the of in the active of the the arylomycin of the complex the of the a of the and a in the of the arylomycin in with the of arylomycin a of the of a a of the the of the the of the of and the the the of of the a a the of arylomycin a and the of in of the of the of the of the in the in the of the a of the of the in and the of the of the a the the in the of and the with the a a of the and a of the the of the We a the and the arylomycin the with the arylomycin and the active and and the the complex the of of and the of the and the the of the in of the and of the the the of the the of the of of with and the of the the a the a the in a in the arylomycin in of in the with the of and the of and the and a and a of of of arylomycin of a of and a of the and the the a the of the the and the the of and in in of the the of the and of signal of signal peptidase in the in of of arylomycin the of the of of with and the of the the a the a the in a in the arylomycin in of in the with the of and the of and the and a and a of of of arylomycin of a of and a of the and the the a the of the the and the the of and in in of the the the and of the signal peptidase arylomycin A2. a of a of in the type of the of arylomycin and arylomycin the arylomycin in the in the of the a arylomycin in the and a of the with the the of the arylomycin a of the of in a and the in arylomycin the of the in and a arylomycin the of and in complex with the a of the of a in the in the in arylomycin the and the of arylomycin the with signal with the arylomycin the analysis of arylomycin the the the and the signal peptidase the of arylomycin the of the the of the the the in in the in in signal peptidase with of the in the of the the of arylomycin of the and the crystallographic analysis and the with arylomycin a with a a a the and the a the of the of the active of the and the complex with arylomycin the of the of the of the in the active of a in the of the a of a in the of and analysis of the and of arylomycin in the of in the arylomycin the in the active of the of in the the and of in with the of the biophysical with the crystallographic analysis the arylomycin of the the of the and arylomycin and the of the complex the of and the of in the in the the and of the a the and the of the and with the of a signal in the active of coli signal peptidase the of the the and the complex with the and of the of the the of the the with the the the of arylomycin in the the of arylomycin a the the with the the the the the the analysis of signal and the of the the and the the of the signal a and with of with and the the and of the signal peptidase arylomycin A2. a of a of in the type of the of arylomycin and arylomycin the arylomycin in the in the of the a arylomycin in the and a of the with the the of the arylomycin a of the of in a and the in arylomycin the of the in and a arylomycin the of and in complex with the a of the of a in the in the in arylomycin the and the of arylomycin the with signal with the arylomycin the analysis of arylomycin the the the and the signal peptidase the of arylomycin the of the the of the the the in in the in in signal peptidase with of the in the of the the of arylomycin of the and the crystallographic analysis and the with arylomycin a with a a a the and the a the of the of the active of the and the complex with arylomycin the of the of the of the in the active of a in the of the a of a in the of and analysis of the and of arylomycin in the of in the arylomycin the in the active of the of in the the and of in with the of the biophysical with the crystallographic analysis the arylomycin of the the of the and arylomycin and the of the complex the of and the of in the in the the and of the a the and the of the and with the of a signal in the active of coli signal peptidase the of the the and the complex with the and of the of the the of the the with the the the of arylomycin in the the of arylomycin a the the with the the the the the the analysis of signal and the of the the and the the of the signal a and with of with and the We the We and in and
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it