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Record W2155823893 · doi:10.1128/jb.01144-10

Genome Sequence of the Obligate Methanotroph <i>Methylosinus trichosporium</i> Strain OB3b

2010· article· en· W2155823893 on OpenAlexaff
Lisa Y. Stein, Sukhwan Yoon, Jeremy D. Semrau, Alan A. DiSpirito, Andrew T. Crombie, J. Colin Murrell, Stéphane Vuilleumier, Marina Kalyuzhnaya, Huub J. M. Op den Camp, Françoise Bringel, David Bruce, Jan‐Fang Cheng, Alex Copeland, Lynne Goodwin, Cliff Han, Loren Hauser, Mike S. M. Jetten, Aurélie Lajus, Miriam Land, Alla Lapidus, Susan Lucas, Claudine Médigue, Samuel Pitluck, Tanja Woyke, Ahmet Zeytun, Martin G. Klotz

Bibliographic record

VenueJournal of Bacteriology · 2010
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMicrobial metabolism and enzyme function
Canadian institutionsUniversity of Alberta
FundersOffice of ScienceJoint Genome InstituteInfrastructures en Biologie Santé et AgronomieUniversity of LouisvilleU.S. Department of EnergyNational Science Foundation
KeywordsMethane monooxygenaseMethanotrophBiologyContext (archaeology)ObligateStrain (injury)Obligate anaerobeMicrobiologyBiochemistryAnaerobic oxidation of methaneGeneticsEnzymeBacteriaBotanyCatalysis

Abstract

fetched live from OpenAlex

Methylosinus trichosporium OB3b (for "oddball" strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.015
Threshold uncertainty score0.029

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.002
Science and technology studies0.0010.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.232
Teacher spread0.222 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations109
Published2010
Admission routes1
Has abstractyes

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