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A New Cytotoxic Carbazole Alkaloid Isolated from the Stem Bark of Malaysian Clausena excavata

Why is this work in the frame?

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

Canadian venueIt was published in a Canadian venue.

No Canadian affiliation. An affiliation-only frame — the usual design — would never have seen this work. It is one of the works that make the case for inverting the frame.

The three-model screen

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All three models called this out of scope.

stratum: venue_new · design weight: 2684.25 (the sample is stratified; any rate computed without the weight is wrong)
Claude Opus 4.8OUT
genre: empirical
about Canada: no
confidence: high

Isolation of a cytotoxic alkaloid from a plant; natural-product chemistry.

GPT-5.6 (high)OUT
genre: empirical
about Canada: no
confidence: high

The study isolates and tests plant compounds for cytotoxicity, not research itself.

Grok 4.5OUT
genre: empirical
about Canada: no
confidence: high

Natural-product chemistry isolation of a cytotoxic alkaloid; domain biomedical chemistry.

Abstract

Abstract: Malaysia is one of the richest in its biodiversity in the world. There are not less than 12,000 plants species in its rain forest. The aim of this study is a continuous investigation for medicinal plants in Malaysia, especially the chemical constituents that are poses significant activities, i.e. anti-cancer. Clausena excavata (Rutaceae) has been known as a very rich in carbazole alkaloids, coumarins and limonoids species. In the present study, one new carbazole alkaloid, 1,8-dihydroxy-3-formyl-4-prenylcarbazole (Clausine–TH), and two other known compounds, Clausenarin (coumarin) and Clausine–K (carbazole alkaloid), were isolated from the methanol extract of the stem bark of Clausena excavata, collected from Kedah, Malaysia. Structures of these compounds were confirmed by various spectroscopic analyses including GC-MS, NMRs, and FTIR. All pure compounds isolated were tested for their cytotoxicity against CEM-SS cell lines, with the Clausine–TH and Clausine–K gave a very strong activity with an IC50 value of 2.1 µg/mL and 5.1 µg/mL, respectively.

Stored with the screening record, where it is evidence for the labels above.

The record

Venue
Canadian Chemical Transactions
Topic
Plant chemical constituents analysis
Field
Agricultural and Biological Sciences
Canadian institutions
Funders
Keywords
AlkaloidCarbazoleCytotoxic T cellBark (sound)ChemistryTraditional medicineStereochemistryOrganic chemistryMedicineForestryGeographyBiochemistryIn vitro
Has abstract in OpenAlex
yes