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Record W2316972784 · doi:10.1107/s0108767308091708

Mechanism for formation of Arg-AMP in help of tRNA on the basis of structure of ArgRS, tRNA and ATP

2008· article· en· W2316972784 on OpenAlexaff
Masae Konno, Emiko Uchikawa, Yusaku Mori, Tomomi Sumida, T. Yanagisawa, Shun‐ichi Sekine, Shigeyuki Yokoyama

Bibliographic record

VenueActa Crystallographica Section A Foundations of Crystallography · 2008
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA and protein synthesis mechanisms
Canadian institutionsUniversity Health Network
Fundersnot available
KeywordsTransfer RNAMechanism (biology)ChemistryBiochemistryRNAPhysicsGene

Abstract

fetched live from OpenAlex

Poster Sessions in the T-state (inactive; NADH bound). Notably, in the unregulated Type I CS these same residues form a stable folded region composed of two large helices that are a key part of the binding site for acetyl-CoA. Kinetic analyses of both types of CS indicate a similar catalytic mechanism, suggesting that in the active R-state of E. coli Type II CS, the conformation of residues 262-298 will be similar to that seen in the unregulated Type I enzymes. This uniquely evolved functionality switch in the Type II CS provides the opportunity to study a novel aspect of the protein folding problem, namely how to engineer the property of reversible instability into a formerly stably folded region. We have specifically designed and determined the structures of variant enzymes to gain a better understanding of the mechanistic features of this allosterically controlled regulatory folding/unfolding system. This has resulted in a series of stepwise structural snap shots of the refolding process in going from the T to R-states in Type II CS.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.018
Threshold uncertainty score0.709

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.235
Teacher spread0.219 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes1
Has abstractyes

Explore more

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