Mechanism for formation of Arg-AMP in help of tRNA on the basis of structure of ArgRS, tRNA and ATP
Bibliographic record
Abstract
Poster Sessions in the T-state (inactive; NADH bound).Notably, in the unregulated Type I CS these same residues form a stable folded region composed of two large helices that are a key part of the binding site for acetyl-CoA.Kinetic analyses of both types of CS indicate a similar catalytic mechanism, suggesting that in the active R-state of E. coli Type II CS, the conformation of residues 262-298 will be similar to that seen in the unregulated Type I enzymes.This uniquely evolved functionality switch in the Type II CS provides the opportunity to study a novel aspect of the protein folding problem, namely how to engineer the property of reversible instability into a formerly stably folded region.We have specifically designed and determined the structures of variant enzymes to gain a better understanding of the mechanistic features of this allosterically controlled regulatory folding/unfolding system.This has resulted in a series of stepwise structural snap shots of the refolding process in going from the T to R-states in Type II CS.Supported by the Natural Sciences and Engineering Research Council of Canada.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.006 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".