Production and Flocculation Conditions of a Novel Glycoprotein Bioflocculant
Bibliographic record
Abstract
The effect of culture conditions on microbial flocculant production from Nocardia sp. CCTCC M201005, a newly isolated bioflocculant producing bacterium, was investigated. Sucrose was proved to be an optimum carbon source. Complexity of organic and inorganic nitrogen sources stimulated both bacterial growth and flocculant synthesis, among which corn steep complexed with urea was the most efficient. Proper corn steep concentration accelerated bacterial growth while relatively low corn steep concentration was more favorable for bioflocculant formation. The highest flocculation activity was obtained at C/N 20~30. The medium supplemented fermentation was designed and carried out. The results showed that the final flocculation activity and cell density were increased more than 10% and 35% respectively compared with those in the fermentation with no medium supplementation. Flocculation conditions of bioflocculant produced by Nocardia sp. CCTCC M201005 (named REA-11) were also discussed. The thermostability of REA-11 was high at pH 3.0 to 6.5 and its activity was markedly improved by the addition of CaCl2. As a co-flocculant, the optimum concentration of CaCl2 was 8 mmol/L in this flocculation system.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".