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Record W2519683881 · doi:10.15252/embj.201693889

FBW7 suppression leads to SOX9 stabilization and increased malignancy in medulloblastoma

2016· article· en· W2519683881 on OpenAlexaff
Aldwin Suryo Rahmanto, Vasil Savov, Andrä Brunner, Sara Bolin, Holger Weishaupt, Alena Malyukova, Gabriela Rosén, Matko Čančer, Sonja Hutter, Anders Sundström, Daisuke Kawauchi, David Jones, Charles Spruck, Michael D. Taylor, Yoon‐Jae Cho, Stefan M. Pfister, Marcel Kool, Andrey Korshunov, Fredrik J. Swartling, Olle Sangfelt

Bibliographic record

VenueThe EMBO Journal · 2016
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicChromatin Remodeling and Cancer
Canadian institutionsSickKids FoundationHospital for Sick Children
FundersInstitute for Molecular Bioscience, University Of QueenslandEuropean Research CouncilSvenska LäkaresällskapetWorldwide Cancer ResearchCancerfondenRadiumhemmets ForskningsfonderUppsala UniversitetScience for Life LaboratoryVetenskapsrådetBarncancerfondenUppsala Multidisciplinary Center for Advanced Computational ScienceSvenska Forskningsrådet FormasKarolinska InstitutetJohns Hopkins UniversityRagnar Söderbergs stiftelseÅke Wiberg Stiftelse
KeywordsBiologyMedulloblastomaMalignancySOX9Cancer researchGeneticsTranscription factorGene

Abstract

fetched live from OpenAlex

Abstract SOX 9 is a master transcription factor that regulates development and stem cell programs. However, its potential oncogenic activity and regulatory mechanisms that control SOX 9 protein stability are poorly understood. Here, we show that SOX 9 is a substrate of FBW 7, a tumor suppressor, and a SCF ( SKP 1/ CUL 1/F‐box)‐type ubiquitin ligase. FBW 7 recognizes a conserved degron surrounding threonine 236 (T236) in SOX 9 that is phosphorylated by GSK 3 kinase and consequently degraded by SCF FBW 7α . Failure to degrade SOX 9 promotes migration, metastasis, and treatment resistance in medulloblastoma, one of the most common childhood brain tumors. FBW 7 is either mutated or downregulated in medulloblastoma, and in cases where FBW 7 mRNA levels are low, SOX 9 protein is significantly elevated and this phenotype is associated with metastasis at diagnosis and poor patient outcome. Transcriptional profiling of medulloblastoma cells expressing a degradation‐resistant SOX 9 mutant reveals activation of pro‐metastatic genes and genes linked to cisplatin resistance. Finally, we show that pharmacological inhibition of PI 3K/ AKT / mTOR pathway activity destabilizes SOX 9 in a GSK 3/ FBW 7‐dependent manner, rendering medulloblastoma cells sensitive to cytostatic treatment.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.023
Threshold uncertainty score0.151

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.250
Teacher spread0.243 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations79
Published2016
Admission routes1
Has abstractyes

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