CRISPR/Cas9 knockouts reveal genetic interaction between strain-transcendent erythrocyte determinants of <i>Plasmodium falciparum</i> invasion
Bibliographic record
Abstract
Significance During malaria infections, Plasmodium falciparum parasites invade RBCs. Identification of host factors for parasite invasion guides the development of vaccines and host-targeted therapeutics. Here we describe the development of an in vitro culture system for the functional analysis of RBC determinants using the immortal erythroleukemia cell line JK-1. JK-1 cells can be induced to differentiate synchronously, support parasite invasion, and are amenable to genetic manipulation. Using this system, we validated two host factors, basigin and CD44, as strain-transcendent host factors for parasite invasion, and we demonstrated a functional interaction between these two proteins. The ability to perform gene editing to produce RBC mutants will augment our ability to study malaria infection.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".