Functional characterization of a reproductive tissue specific promoter from <i>Eucalyptus camaldulensis</i>
Bibliographic record
Abstract
SWEET proteins are essential for the maintenance of nectar production, as well as seed and pollen development, in plants. A search within the Eucalyptus genome identified 52 putative genes belonging to the SWEET gene family based on sequence similarity. The expression of two of these genes, EcSWEET2 and EcSWEET5, was analyzed in vegetative and reproductive tissues of Eucalyptus camaldulensis. The expression of EcSWEET5 was specific to male reproductive tissues, and transcripts were detected only at certain stages of flower development. Tobacco Rattle Virus (TRV)-mediated suppression of EcSWEET5 resulted in a significant reduction in pollen germination percentage in Nicotiana benthamiana without adverse effect on vegetative growth. A promoter sequence 1 kb upstream of the start codon of EcSWEET5 contained many elements suggestive of pollen specificity of the promoter. This specificity was confirmed in transgenic tobacco lines harboring a GUS gene whose expression was controlled by the EcSWEET5 gene promoter. GUS expression was limited to pollen alone in transgenic tobacco as evidenced by histochemical staining. The expression of a cytotoxic gene, barnase under the control of the EcSWEET5 gene promoter, showed pollen ablation in transgenic tobacco with normal vegetative growth.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".