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Record W2917521450 · doi:10.1073/pnas.1814711116

NOTCH1 signaling induces pathological vascular permeability in diabetic retinopathy

2019· article· en· W2917521450 on OpenAlexafffund
Khalil Miloudi, Malika Oubaha, Catherine Ménard, Agnieszka Dejda, Vera Guber, Gaël Cagnone, Ariel M. Wilson, Nicolas Tétreault, Gaëlle Mawambo, François Binet, Rony Chidiac, Chantal Delisle, Manuel Buscarlet, Agustin Cerani, Sergio Crespo‐Garcia, Katie Bentley, Flávio Rezende, Jean‐Sébastien Joyal, Frédérick A. Mallette, Jean‐Philippe Gratton, Bruno Larrivée, Przemysław Sapieha

Bibliographic record

VenueProceedings of the National Academy of Sciences · 2019
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRetinal Development and Disorders
Canadian institutionsCentre Hospitalier Universitaire Sainte-JustineMcGill UniversityUniversité de MontréalHôpital Maisonneuve-Rosemont
FundersCanadian Institutes of Health ResearchNatural Sciences and Engineering Research Council of CanadaFoundation Fighting BlindnessCanadian Diabetes AssociationGovernment of CanadaHeart and Stroke Foundation of Canada
KeywordsDiabetic retinopathyVascular permeabilityDiabetes mellitusMedicineEndothelial dysfunctionNitric oxidePathologicalNotch signaling pathwayBlindnessVE-cadherinEndotheliumRetinopathySignal transductionCancer researchBioinformaticsInternal medicineCell biologyEndocrinologyReceptorBiologyEndothelial stem cellBiochemistry

Abstract

fetched live from OpenAlex

Diabetic macular edema is a major complication of diabetes resulting in loss of central vision. Although heightened vessel leakiness has been linked to glial and neuronal-derived factors, relatively little is known on the mechanisms by which mature endothelial cells exit from a quiescent state and compromise barrier function. Here we report that endothelial NOTCH1 signaling in mature diabetic retinas contributes to increased vascular permeability. By providing both human and mouse data, we show that NOTCH1 ligands JAGGED1 and DELTA LIKE-4 are up-regulated secondary to hyperglycemia and activate both canonical and rapid noncanonical NOTCH1 pathways that ultimately disrupt endothelial adherens junctions in diabetic retinas by causing dissociation of vascular endothelial-cadherin from β-catenin. We further demonstrate that neutralization of NOTCH1 ligands prevents diabetes-induced retinal edema. Collectively, these results identify a fundamental process in diabetes-mediated vascular permeability and provide translational rational for targeting the NOTCH pathway (primarily JAGGED1) in conditions characterized by compromised vascular barrier function.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.270
Teacher spread0.248 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations90
Published2019
Admission routes2
Has abstractyes

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