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Record W2963839877 · doi:10.1038/s41467-019-11306-6

FDA-ARGOS is a database with public quality-controlled reference genomes for diagnostic use and regulatory science

2019· article· en· W2963839877 on OpenAlexfundno aff
Heike Sichtig, Timothy D. Minogue, Yi Yan, Christopher P. Stefan, Adrienne T. Hall, Luke J. Tallon, Lisa Sadzewicz, Suvarna Nadendla, William Klimke, Eneida Hatcher, Martin Shumway, Dayanara Lebron Aldea, Jonathan Allen, Jeffrey W. Koehler, Tom Slezak, Stephen Lovell, Randal J. Schoepp, Uwe Scherf

Bibliographic record

VenueNature Communications · 2019
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCRISPR and Genetic Engineering
Canadian institutionsnot available
FundersU.S. Army Medical Research Institute of Infectious DiseasesU.S. National Library of MedicineUniversity of Colorado School of Medicine, Anschutz Medical CampusDHHS Office of the SecretaryDefense Threat Reduction AgencyHamilton Health Sciences FoundationPublic Health AgencyChildren's National HospitalBritish Columbia Centre for Disease ControlPublic Health EnglandNational Institutes of HealthU.S. Department of Health and Human ServicesU.S. Department of EnergyU.S. Department of Defense
KeywordsComputer scienceQuality (philosophy)GenomeDatabaseComputational biologyBiologyGeneticsGene

Abstract

fetched live from OpenAlex

FDA proactively invests in tools to support innovation of emerging technologies, such as infectious disease next generation sequencing (ID-NGS). Here, we introduce FDA-ARGOS quality-controlled reference genomes as a public database for diagnostic purposes and demonstrate its utility on the example of two use cases. We provide quality control metrics for the FDA-ARGOS genomic database resource and outline the need for genome quality gap filling in the public domain. In the first use case, we show more accurate microbial identification of Enterococcus avium from metagenomic samples with FDA-ARGOS reference genomes compared to non-curated GenBank genomes. In the second use case, we demonstrate the utility of FDA-ARGOS reference genomes for Ebola virus target sequence comparison as part of a composite validation strategy for ID-NGS diagnostic tests. The use of FDA-ARGOS as an in silico target sequence comparator tool combined with representative clinical testing could reduce the burden for completing ID-NGS clinical trials.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.016
metaresearch head score (Gemma)0.032
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Dataset · Consensus signal: Dataset
Teacher disagreement score0.018
Threshold uncertainty score0.085

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0160.032
Meta-epidemiology (narrow)0.0020.001
Meta-epidemiology (broad)0.0020.001
Bibliometrics0.0100.010
Science and technology studies0.0010.001
Scholarly communication0.0040.003
Open science0.0040.003
Research integrity0.0030.002
Insufficient payload (model declined to judge)0.0180.012

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.031
GPT teacher head0.353
Teacher spread0.322 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreDataset

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations332
Published2019
Admission routes1
Has abstractyes

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