Secondary Metabolite Biosynthesis during Vegetative Incompatibility in Cryphonectria parasitica, Causal Agent of the American Chestnut Blight
Bibliographic record
Abstract
Vegetative Incompatibility (VI) can occur when filamentous fungi fuse during the asexual (mitotic) growth phase. In Cryphonectria parasitica, VI is modulated by six vic loci; hyphal fusion between strains that differ at one or more vic loci results in cell death. VI is considered an immune-like response that restricts heterokaryon formation and transmission of deleterious cytoplasmic elements through unknown mechanisms. A transcriptomic analysis of vic3associated incompatibility identified a set of differentially expressed genes within biosynthetic gene clusters (BGCs). Using UPLC-HRMS, we detected significant shifts in secondary metabolite production during vic3 incompatibility. Among these metabolites, HPLC purification and NMR structure elucidation revealed that a new variant of calbistrin is linked to VIupregulated BGCs, as is a farnesyl-S-oxide analog resembling mating pheromones. Similarly, detection of putative lysophosphatidylcholines and other modified lipids implicates membrane alteration during VI. Together, these findings suggest VI involves synthesis of novel secondary metabolites and complex membrane-associated signaling.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".