Tissue Plasminogen Activator (tPA) Promotes Postnatal Cortical Neuron Survival in Vitro Via JAK2- and mTOR-Dependent Mechanisms
Bibliographic record
Abstract
Tissue plasminogen activator (tPA) is the only approved drug for ischemic stroke in Canada but is limited in its clinical efficacy due to its short therapeutic window.This study sought to determine the effect of tPA on postnatal primary cortical neuron viability and aimed to identify the relevant cellular signalling mechanisms underlying the tPA-mediated effects in vitro.The data revealed that tPA significantly increased the propensity for cell survival within a time latency window of up to 3 hours.tPA-induced neuroprotective effects were significantly dependent upon the mTOR and JAK/STAT signalling pathways, while the MEK and PKA signalling pathways were found to play a less critical role.Immunocytochemical staining showed a marked increase in p-S6 expression following treatment with tPA, substantiating the vital role of mTOR activation in tPA-mediated neuroprotection.These results suggest the possibility of targeting the defined mechanisms to expand the therapeutic window of tPA in stroke recovery.tPA PROMOTES POSTNATAL CORTICAL NEURON SURVIVAL iii
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".