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Record W3088365751 · doi:10.1101/2020.09.24.311498

Human marginal zone B cell development from early T2 progenitors

2020· preprint· en· W3088365751 on OpenAlexaff
Thomas J Tull, Michael J. Pitcher, William Guesdon, Jacqueline H. Siu, Cristina Lebrero‐Fernández, Yuan Zhao, Nedyalko Petrov, Susanne Heck, Richard J. Ellis, Pawan Dhami, Ulrich D. Kadolsky, Michelle Kleeman, Yogesh Kamra, David J. Fear, Susan John, Wayel Jassem, Richard Groves, Jeremy Sanderson, Michael D. Robson, David D’Cruz, Mats Bemark, Jo Spencer

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2020
Typepreprint
Languageen
FieldImmunology and Microbiology
TopicT-cell and B-cell Immunology
Canadian institutionsSt. Thomas Hospital
FundersMedical Research CouncilVetenskapsrådetNational Institute for Health and Care Research
KeywordsMarginal zoneBiologyBone marrowImmunologyB cellProgenitor cellHoming (biology)HaematopoiesisStem cellCell biologyReceptorMolecular biologyAntibodyGenetics

Abstract

fetched live from OpenAlex

Abstract B cells emerge from the bone marrow as transitional (TS) B cells that differentiate through T1, T2 and T3 stages to become naïve B cells. We have identified a bifurcation of human B cell maturation from the T1 stage forming IgM hi and IgM lo developmental trajectories. IgM hi T2 cells have higher expression of α4β7 integrin and lower expression of IL4 receptor (IL4R) compared to the IgM lo branch and are selectively recruited into gut-associated lymphoid tissue. IgM hi T2 cells also share transcriptomic features with marginal zone B cells (MZB). Lineage progression from T1 cells to MZB via an IgM hi trajectory is identified by pseudotime analysis of scRNA-sequencing data. Reduced frequency of IgM hi gut homing T2 cells is observed in severe SLE and is associated with reduction of MZB and their putative IgM hi precursors. The collapse of the gut-associated MZB maturational axis in severe SLE affirms its existence and importance for maintaining health.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.204
Teacher spread0.189 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations9
Published2020
Admission routes1
Has abstractyes

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