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Record W3088401235 · doi:10.1038/s41586-020-2797-4

Cells of the adult human heart

2020· article· en· W3088401235 on OpenAlexafffund
Monika Litviňuková, Carlos Talavera‐López, Henrike Maatz, Daniel Reichart, Catherine L. Worth, Eric L. Lindberg, Masatoshi Kanda, Krzysztof Polański, Matthias Heinig, Michael Lee, Emily R. Nadelmann, Kenny Roberts, Elizabeth Tuck, Eirini S. Fasouli, Daniel M. DeLaughter, Barbara McDonough, Hiroko Wakimoto, Joshua Gorham, Sara Samari, Krishnaa T. Mahbubani, Kourosh Saeb‐Parsy, Giannino Patone, Joseph J. Boyle, Hongbo Zhang, Hao Zhang, Anissa Viveiros, Gavin Y. Oudit, Omer Ali Bayraktar, Jonathan G. Seidman, Christine E. Seidman, Michela Noseda, Norbert Hübner, Sarah A. Teichmann

Bibliographic record

VenueNature · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSingle-cell and spatial transcriptomics
Canadian institutionsUniversity of Alberta
FundersNational Institutes of HealthAlberta InnovatesChina Scholarship CouncilBundesministerium für Bildung und ForschungDeutsches Zentrum für Herz-KreislaufforschungEuropean CommissionUniversity of AlbertaDeutsche ForschungsgemeinschaftSarnoff Cardiovascular Research FoundationBritish Heart FoundationHoward Hughes Medical InstituteChan Zuckerberg InitiativeAlexander von Humboldt-StiftungWellcome TrustNational Heart, Lung, and Blood InstituteHeart and Stroke Foundation of CanadaCanadian Institutes of Health ResearchNational Science Foundation
KeywordsTranscriptomeBiologyCell typeMyocyteImmune systemCellNeuroscienceHuman heartCardiac muscleCell biologyCardiac VentricleHeart diseaseGene expressionMedicinePathologyAnatomyGeneImmunologyInternal medicineGenetics

Abstract

fetched live from OpenAlex

Cardiovascular disease is the leading cause of death worldwide. Advanced insights into disease mechanisms and therapeutic strategies require a deeper understanding of the molecular processes involved in the healthy heart. Knowledge of the full repertoire of cardiac cells and their gene expression profiles is a fundamental first step in this endeavour. Here, using state-of-the-art analyses of large-scale single-cell and single-nucleus transcriptomes, we characterize six anatomical adult heart regions. Our results highlight the cellular heterogeneity of cardiomyocytes, pericytes and fibroblasts, and reveal distinct atrial and ventricular subsets of cells with diverse developmental origins and specialized properties. We define the complexity of the cardiac vasculature and its changes along the arterio-venous axis. In the immune compartment, we identify cardiac-resident macrophages with inflammatory and protective transcriptional signatures. Furthermore, analyses of cell-to-cell interactions highlight different networks of macrophages, fibroblasts and cardiomyocytes between atria and ventricles that are distinct from those of skeletal muscle. Our human cardiac cell atlas improves our understanding of the human heart and provides a valuable reference for future studies.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.009
Threshold uncertainty score0.032

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.001
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0090.010

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.224
Teacher spread0.217 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1,771
Published2020
Admission routes2
Has abstractyes

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