MOLECULAR DETECTION OF SALMONELLA AND E. COLI MICROORGANISMS AMONG DAIRY FARMS WITH DETECTION OF VIRULENCE AND ANTIBIOTICS RESISTANCE GENES
Bibliographic record
Abstract
A total of 500 samples, 100 of each milk, feed, swabs from milking equipment (milk tanks), drinking tanks swabs and dairy cows fecal swabs samples were collected from different small herds of apparently or subclinical dairy cattle in El-Kabotti and Bahr El-Baker zone at Port-Said Governorates during the period from September to December 2018. The samples were examined for isolation and identification of Salmonella species and E. coli with studied of their virulence and resistance gens and sequence of some genes. The results revealed that Salmonella species and E. coli could be detected in a percentage of 1.8% and 2.8% respectively from the examined samples. Salmonella isolates from the examined samples were identified biochemically and serological as S. Typhimurium S. Entiriditis and S. saintipaul with a percentage of 66.67% (6/9), 2.22% (2/9) and 11.11% (1/9) respectively, while that of E. coli were O26 (5/14), O119 (2/14), O125 (4/14), O126 (1/14) and O127 (2/14) with a percentage of 35.71%, 14.28%, 28.60%, 7.14% and 14.28% respectively. The isolated strains of Salmonella species (n=9) and E. coli stains (N=14) were investigated for antibiotic susceptibility profile to 10 antibacterial agents by disc diffusion method. The resistances of the isolated Salmonella and E. coli strains were ranged from a various degree of resistances to complete resistances (100%). By using conventional PCR, all Salmonella were harbored InvA, stn and bcfC genes while E. coli were harbored PhoA, TraT and fimH genes. The resistance genes that detected in Salmonella strains were ampC, mphA and aacC while that of E. coli were bltEm, ampC, mphA, Aada1 and aacC. The prevalence of the resistance genes were discussed. DNA sequencing of stn and bcfC genes for Salmonella and TraT and fimH genes for E. coli were discussed and compared with other strains in Gen Bank. The mutations in quinolone-resistance gene were studied by determining regions of the gyrA gene for Salmonella and E. coli. The public health hazards of these microorganisms as well as recommended measures to improve hygiene measures in dairy farms were discussed.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".