S. Aureus Lipoteichoic Acid Failed to Activate NLRP3, but Downregulated the Transduction of NLRP3/MAPK and NF-κB Signaling Pathway in Mouse Mastitis Induced by S. Aureus
Bibliographic record
Abstract
Abstract Background It was confirmed that S. aureus infection activated the expression of NLRP3. As a major component of S. aureus cell wall, what about LTA? Firstly, in this study a mouse model was built following LTA intramammary infection for 0, 12, 24, 48 and 72 h, the characterization of mammary inflammatory response induced by LTA was observed. Subsequently, mouse mammary preconditioned with LTA was modelling. The effect of LTA treatment on the activation of NLRP3/ MAPK and NF-κB signaling pathways induced by S. aureus were detected. Results Results shown that the LTA intramammary infection induce mild but rapid recovery inflammation in mammary gland whereas failed to stimulate NLRP3. LTA treatment protected mammary gland against S. aureus infection by suppressed the activation of NLRP3/ MAPK and NF-κB signaling pathways. Meanwhile it is noteworthy that the expression of NLRP3/MAPK JNK induced by S. aureus was not regulated by LTA treatment. Conclusions These results suggested LTA induced mammary gland inflammation was mild and self-limiting. LTA treatment revealed a good anti-inflammation effect through downregulating the transduction of NLRP3/MAPK and NF-κB signaling pathways in mouse mastitis induced by S. aureus . Our research highlights the importance of an LTA during the innate immune response of the mammary gland and offer novel insights for new approaches concerning effective immunomodulation against a local bacterial infection.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.011 | 0.004 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.003 | 0.003 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.002 | 0.002 |
| Research integrity | 0.002 | 0.009 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".