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Record W4380415292 · doi:10.1021/acs.est.3c00159

Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples

2023· article· en· W4380415292 on OpenAlexafffund
Xiaole Yin, Xi Chen, Xiaotao Jiang, Ying Yang, Bing Li, Marcus Ho-Hin Shum, Tommy Tsan‐Yuk Lam, GM Leung, Joan B. Rose, Concepcion Sanchez-Cid, Timothy M. Vogel, Fiona Walsh, Thomas U. Berendonk, Janet Midega, Chibuzor Uchea, Dominic Frigon, Gerard D. Wright, Cornelius Carlos Bezuidenhout, Renata Cristina Picão, Shaikh Ziauddin Ahammad, Per Halkjær Nielsen, Philip Hugenholtz, Nicholas J. Ashbolt, Gianluca Corno, Despo Fatta‐Kassinos, Helmut Bürgmann, Heike Schmitt, Chang‐Jun Cha, Amy Pruden, Kornelia Smalla, Eddie Cytryn, Yu Zhang, Min Yang, Yong‐Guan Zhu, Arnaud Dechesne, Barth F. Smets, David W. Graham, Michael R. Gillings, William H. Gaze, Célia M. Manaia, Mark C.M. van Loosdrecht, Pedro J. J. Alvarez, Martin J. Blaser, James M. Tiedje, Edward Topp, Tong Zhang

Bibliographic record

VenueEnvironmental Science & Technology · 2023
Typearticle
Languageen
FieldEnvironmental Science
TopicPharmaceutical and Antibiotic Environmental Impacts
Canadian institutionsMcMaster UniversityAgriculture and Agri-Food CanadaMcGill University
FundersEidgenössische Anstalt für Wasserversorgung Abwasserreinigung und GewässerschutzRijksinstituut voor Volksgezondheid en MilieuNatural Environment Research CouncilMedical Research CouncilTsinghua Shenzhen International Graduate SchoolUniversidade Federal do Rio de JaneiroCollege of Engineering, Michigan State UniversityIndian Institute of Technology DelhiUniversity of CyprusTechnische Universität DresdenUniversity of New South WalesChung-Ang UniversityAalborg UniversitetEngineering and Physical Sciences Research CouncilTechnische Universiteit DelftUniversité de LyonSun Yat-sen UniversityUniversity of QueenslandCentre National de la Recherche ScientifiqueSouthern Cross UniversityUniversity of Hong KongSight Research UKMichigan State UniversityTsinghua UniversityNorth-West UniversityResearch Grants Council, University Grants CommitteeWellcome TrustMcGill UniversityMcMaster University
KeywordsBiologyAntibiotic resistanceComparabilityGeneHuman healthComputational biologyCopy-number variationBiotechnologyGenomeGeneticsAntibioticsMedicineEnvironmental health

Abstract

fetched live from OpenAlex

Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys in human and animal sectors under the "One-Health" framework. However, there are substantial challenges in comparing and synthesizing the results of multiple studies that employ different test methods and approaches in bioinformatic analysis. In this article, we consider the commonly used quantification units (ARG copy per cell, ARG copy per genome, ARG density, ARG copy per 16S rRNA gene, RPKM, coverage, PPM, etc.) for profiling ARGs and suggest a universal unit (ARG copy per cell) for reporting such biological measurements of samples and improving the comparability of different surveillance efforts.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Science and technology studies
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.233
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.002
Science and technology studies0.0000.005
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.048
GPT teacher head0.288
Teacher spread0.241 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations157
Published2023
Admission routes2
Has abstractyes

Explore more

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