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P1473: CLINICALLY RELEVANT HEMOGLOBIN RESPONSE IN ADULTS WITH PYRUVATE KINASE DEFICIENCY TREATED WITH MITAPIVAT – A SUB-ANALYSIS OF THE ACTIVATE TRIAL

2023· article· en· W4385856750 on OpenAlexaff
Hanny Al‐Samkari, Rachael F. Grace, Andreas Glenthøj, Wilma Barcellini, Madeleine Verhovsek, Jennifer Rothman, Marta Morado, Mark Layton, Oliver Andrés, F. Galactéros, Koichi Onodera, Satheesh Chonat, Rengyi Xu, Bryan McGee, Melissa Dibacco, Jaime Morales, Eduard J. van Beers

Bibliographic record

VenueHemaSphere · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBiochemical and Molecular Research
Canadian institutionsMcMaster University
Fundersnot available
KeywordsMedicineAnemiaHemoglobinHemolysisClinical endpointPyruvate kinasePlaceboPyruvate kinase deficiencyInternal medicineRandomized controlled trialGastroenterologyClinical trialPharmacologyGlycolysisPathologyMetabolism

Abstract

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Topic: 28. Enzymopathies, membranopathies and other anemias Background: Mitapivat, an oral, allosteric activator of pyruvate kinase (PK), is approved by the US FDA for the treatment of hemolytic anemia in adults with PK deficiency and by the EMA for the treatment of PK deficiency in adults. In the ACTIVATE trial (NCT03548220), mitapivat demonstrated improvements in hemoglobin (Hb) in patients (pts) who were not regularly transfused. 16/40 (40%) pts receiving mitapivat met the primary endpoint of Hb response (≥1.5 g/dL increase from baseline [BL] sustained at ≥2 scheduled assessments at weeks [wks] 16, 20, and 24 in the fixed-dose period) compared with 0 for placebo (PBO). Understanding Hb response using a clinically applicable definition of ≥1.0 g/dL improvement after mitapivat treatment may provide additional benefits. Aims: This analysis examined Hb, hemolysis, and disease-specific patient-reported outcome (PRO) responses to mitapivat during ACTIVATE and/or the long-term extension (LTE) (NCT03853798) in pts who had a ≥1.0 g/dL Hb increase. Methods: In the global, phase 3, randomized, PBO-controlled ACTIVATE trial, 80 pts were randomized 1:1 to receive mitapivat or PBO for a 12-wk dose-optimization period (5/20/50 mg twice daily), followed by a 12-wk fixed-dose period. This analysis included pts treated with mitapivat in ACTIVATE who continued into the LTE and had a clinically relevant Hb response defined as ≥1.0 g/dL increase from BL for at least 2 timepoints after the start of the fixed-dose period. Hemolysis was evaluated through bilirubin and reticulocyte analyses. Quality of life was evaluated by using the PRO measures, PK deficiency diary (PKDD) and PK deficiency impact assessment (PKDIA); for both, a lower score represents lower disease burden. The minimal clinically important change (MCIC) is defined as a reduction of 4.2 and 5.5 in PKDD and PKDIA scores, respectively. Results: 25/40 (62.5%) pts originally randomized to mitapivat in ACTIVATE/LTE met the criteria for having a clinically relevant Hb response (≥1.0 g/dL) which occurred as early as 20 wks and as late as 108 wks. Median (Q1, Q3) Hb improvement from BL to Wk 108 was 2.6 g/dL (1.17, 3.27; n=19) (Figure). This group included a subset of 9 pts who did not meet original protocol endpoint (≥1.5 g/dL increase in Hb). For this subset, median (Q1, Q3) Hb improvement from BL at Wk 108 was 1.07 g/dL (0.69,1.92; n=8). 19/25 pts meeting the criteria for clinically relevant Hb response had a ≥1.5 g/dL Hb increase on ≥2 timepoints after the start of the fixed-dose period, of which 16 achieved this by Wk 24; 3 pts achieved ≥1.5 g/dL Hb improvement after Wk 24 (up to Wk 120). Median (Q1, Q3) change from BL to Wk 108 in bilirubin was -30.1 µmol/L (-55.15, -19.20; n=17) and in reticulocyte % was -8.3% (-19.2%, -2.8%; n=18). Improvements in hemolysis markers were similar in the subset of 9 who did not meet original protocol endpoint. At Wk 108, mean (95% CI) changes from BL in PKDD and PKDIA scores were -7.2 (-11.1, -3.2; n=15) and -7.3 (-10.8, -3.8; n=17), respectively (Figure). For the subset of 9 pts, mean (95% CI) changes from BL in PKDD and PKDIA scores were -6.1 (-14.6, 2.3; n=4) and -6.2 (-15.5, 3.2; n=6), respectively. Summary/Conclusion: This analysis shows that a majority of pts treated with mitapivat in ACTIVATE/LTE (62.5%) achieved a clinically relevant Hb response, defined as a Hb increase of ≥1.0 g/dL, along with improvements in hemolysis and PROs, indicating beneficial effects to pts with PK deficiency. Further, some Hb responses ≥1.0 g/dL occurred after 6 months, indicating that certain pts may reach this threshold with continued treatment regardless of initial Hb response.Keywords: Hemolysis, Pyruvate kinase deficiency, Hemolytic anemia, Hemoglobin

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How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.143
Threshold uncertainty score0.527

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.002
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.272
Teacher spread0.260 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2023
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