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Record W4386453085 · doi:10.1080/01691864.2023.2252098

Robotic denudation of zygotes

2023· article· en· W4386453085 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueAdvanced Robotics · 2023
Typearticle
Languageen
FieldMedicine
TopicReproductive Biology and Fertility
Canadian institutionsUniversity of Toronto
FundersChina Oxford Scholarship FundNational Natural Science Foundation of China
KeywordsZygoteDenudationVitrificationPipetteBiologyEmbryoBiological systemComputer scienceCell biologyChemistryAndrologyEmbryogenesisTectonicsPaleontology

Abstract

fetched live from OpenAlex

The vitrification technology is used in the embryo freezing process. To ensure intracellular water removal and avoid ice crystal formation, the cumulus cells surrounding zygotes need to be removed. As operators have varying skill and long-time fatigue, it is challenging to completely remove the cumulus cells surrounding zygotes and reduce zygote loss inside the micropipette. The objective of this work is to develop a robotic zygote denudation system for the vitrification procedure. The robotic system enables accurate segmentation of cumulus cells for estimating the mass of cumulus zygote complexes (CZCs) inside the micropipette via the Res-Unet neural network. A mathematical model was built to describe the dynamic motion of CZCs inside the micropipette, and a model-based optimal controller was developed to aspirate and deposit CZCs inside the micropipette. To denude zygote completely, the Res-Attention neural network was used to detect cumulus cells for predicting the quality of zygote denudation. In the mouse zygotes experiments, the yield rate is 98.2% ± 1.6% and the denudation efficacy is 96.9% ± 0.2% for the robotic system. Compared with manual denudation, the survival rate and development rate of embryos cultured from zygotes denuded by the robotic system were both higher in subsequent vitrification procedure.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.693
Threshold uncertainty score0.216

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.031
GPT teacher head0.317
Teacher spread0.286 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it