Bradyrhizobium ontarionense sp. nov. isolated from Aeschynomene indica (Indian jointvetch) harbours photosynthesis, nitrogen fixation and nitrous oxide reductase genes.
Bibliographic record
Abstract
Abstract Bacterial strain A19T was previously isolated from a root-nodule of Aeschynomene indica (Indian jointvetch) and assigned to a new lineage in the genus Bradyrhizobium. Here data are presented for the detailed phylogenomic and taxonomic characterisation of strain A19T. Phylogenetic analysis of whole genome sequences as well as 51 concatenated core gene sequences placed strain A19T in a highly supported lineage that was distinct from described Bradyrhizobium species; B. oligotrophicum, a symbiont of A. indica, was the most closely related species. The digital DNA-DNA hybridization and average nucleotide identity values for strain A19T in pair-wise comparisons with close relatives were far lower than the respective threshold values of 70% and ~96% for definition of species boundaries. The complete genome of strain A19T consists of a single 8.44 Mbp chromosome (DNA G+C content, 64.9 mol%) and contains a photosynthesis gene cluster, nitrogen-fixation genes and genes encoding a complete denitrifying enzyme system including nitrous oxide reductase. Nodulation and type III secretion system genes, needed for nodulation by most rhizobia, were not detected in the genome of A19T. Data for multiple phenotypic tests complemented the sequence-based analyses. Strain A19T elicits nitrogen-fixing nodules on stems and roots of A. indica plants but not on soybeans or Macroptilium atropurpureum. Based on the data presented, a new species named Bradyrhizobium ontarionense sp. nov. is proposed with strain A19T (= LMG 32638T = HAMBI 3761T ) as the type strain.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".