The host protein CCT8 interacts with PB2 protein of H9N2 AIV and facilitates virus replication
Bibliographic record
Abstract
In recent years, the poultry industry and public health safety have been seriously threatened by low pathogenic avian influenza virus (AIV), such as H9N2. PB2, as a member of RNA polymerase complex of avian influenza virus, plays a crucial role in viral replication and the infection process. To elucidate the mechanism of H9N2 influenza virus infection in host cells, PB2 protein was used as target protein to screen the interacting host proteins through CO-IP, silver staining, and protein mass spectrometry. It was identified that chaperonin containing TCP-1 subunit 8 (CCT8) might interact with PB2 protein during viral replication. H9N2 subtype AIV infection induces the upregulation of endogenous CCT8 gene mRNA and protein levels in cells. Knockdown of CCT8 using siRNA resulted in decreased expression at both the protein and mRNA levels for viral PB2 and NS1 within infected cells, leading to a corresponding reduction in progeny virus production. Conversely, the overexpression of CCT8 increased the protein expression of PB2 and NS1, and also enhanced the mRNA levels of PB2 and NS1 genes. Furthermore, the production of progeny viruses was accordingly increased upon overexpression of CCT8 in 293 T cells. These findings highlight the crucial role played by CCT8 in viral replication, providing novel insights for developing strategies to prevent and treat AIV infection.RESEARCH HIGHLIGHTS The host protein CCT8 interacts with the influenza virus protein PB2.The CCT8 gene is upregulated during influenza virus infection.Knockdown of CCT8 inhibits viral proliferation.Elevated expression of CCT8 facilitates viral proliferation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".