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Record W4410334635 · doi:10.1111/jcmm.70569

RETRACTED: Sex‐Dependent Paracrine Effect of Conditioned Media From Adipose Tissue Derived Mesenchymal Stem Cells on Prostate Cancer Cells

2025· article· en· W4410334635 on OpenAlexaff
Akram Mirzaei, Rahil Mashhadi, Ziba Aghsaeifard, Seyedeh Negin Hashemi Dougaheh, Reza Omid, Fateme Guitynavard, Parsa Nikoofar, Seyed Mohammad Kazem Aghamir

Post-publication record

OpenAlex flags this work as retracted, but it carries no matching Retraction Watch record in this frame.

Bibliographic record

VenueJournal of Cellular and Molecular Medicine · 2025
Typearticle
Languageen
FieldMedicine
TopicTissue Engineering and Regenerative Medicine
Canadian institutionsThunder Bay Regional Research Institute
Fundersnot available
KeywordsParacrine signallingMesenchymal stem cellProstate cancerVimentinEpithelial–mesenchymal transitionCancer researchBiologyAdipose tissueCancer cellCancerApoptosisAngiogenesisMedicineInternal medicineMetastasisImmunologyCell biologyEndocrinologyImmunohistochemistry

Abstract

fetched live from OpenAlex

ABSTRACT Considering the different behaviour of cells in response to diseases in different conditions and sex hormone‐dependent cancers, we addressed the possible effect of the sex of the source of these cells from adipose tissue on prostate cancer cells. In this in vitro study, we evaluated the effects of male and female MSC Conditioned Media (MCM, FCM) on prostate cancer cells. The assessment included Hoechst dye staining, a scratch‐wound assay, a colony formation assay, and a flow cytometric analysis of apoptosis and the cell cycle. We also performed real‐time PCR to examine various genes, including apoptosis‐related genes, epithelial‐mesenchymal transition ( EMT ) genes, angiogenesis‐related genes, and cell growth and survival biomarkers. Our results indicated that the IC 50 values were 50% and 75% media in MCM and FCM in each of the three prostate cancer cell lines, respectively. An evaluation of gene expression revealed that in all three prostate cancer cell lines, treatment with MCM was more effective than FCM in reducing the expression of N‐Cadherin and Vimentin , EGFR and BCL2 genes ( p < 0.001). Furthermore, the MCM significantly increased the expression of BAX and E‐Cadherin genes ( p < 0.001) in the PC3 cell line. MCM proved to be more effective than FCM in reducing the expression of the epithelial‐mesenchymal transition pathway, EGFR gene, and Apoptosis Regulator ( BCL2 ) in the PC3 cell line. Due to its potential in regenerative medicine and cell therapy, this approach may serve as an effective treatment option for advanced prostate cancer.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesResearch integrity
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score1.000
Threshold uncertainty score0.013

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0040.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.257
Teacher spread0.251 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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