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Record W4411882220 · doi:10.1038/s41467-025-60699-0

Prevalent chromosome fusion in Vibrio cholerae O1

2025· article· en· W4411882220 on OpenAlexafffund
Aline Cuénod, Denise Chac, Ashraful Islam Khan, Fahima Chowdhury, Randy W. Hyppa, Susan M. Markiewicz, Amelia Rice, Akhil Kholwadwala, Stephen B. Calderwood, Edward T. Ryan, Jason B. Harris, Regina C. LaRocque, Taufiqur Rahman Bhuiyan, Gerald R. Smith, Firdausi Qadri, Patrick Lypaczewski, Ana A. Weil, B. Jesse Shapiro

Bibliographic record

VenueNature Communications · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicVibrio bacteria research studies
Canadian institutionsMcGill Genome CentreMcGill University Health CentreMcGill University
FundersEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNational Institute of Allergy and Infectious DiseasesNational Institute of General Medical SciencesCanadian Institutes of Health ResearchNational Science FoundationNational Institutes of HealthSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
KeywordsVibrio choleraeBiologyChromosomeVibrionaceaeGeneticsCholeraVirulencePathogenPhenotypeEl TorHomologous chromosomeMicrobiologyGeneBacteria

Abstract

fetched live from OpenAlex

Two circular chromosomes are a defining feature of the bacterial family Vibrionaceae, including the pathogen Vibrio cholerae, with rare reports of isolates with a single, fused chromosome. Here, we use long-read sequencing to analyse 467 V. cholerae O1 isolates from 47 cholera patients and household contacts in Bangladesh. We identify several independent chromosome fusion events that are likely transmissible within a household. Fusions occur in a 12 kilobase-pair homologous sequence shared between the two chromosomes and are stable for at least 200 generations under laboratory conditions. We find no detectable effect of fusion on V. cholerae growth, virulence factor expression, or biofilm formation. The factors promoting fusion, affecting chromosome stability, and subtle phenotypic or clinical consequences merit further investigation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.917
Threshold uncertainty score0.384

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.339
Teacher spread0.325 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2025
Admission routes2
Has abstractyes

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