Zyxin directly binds to chromosomal DNA and is linked with mitochondrial integrity and apoptosis
Bibliographic record
Abstract
Human zyxin is a key component of the focal adhesion complex, playing a role in mediating cell-cell adhesion and cytoskeletal dynamics. Moreover, zyxin shuttles between the cytoplasm and the nucleus, where it contributes to regulating gene expression. Although identified over 30 years ago, zyxin's nuclear functions, particularly in relation to cancer, remain largely unexplored. In this study, we profiled zyxin binding to chromosomal DNA using metastatic prostate carcinoma PC3M cells as a model. Our ChIP-Seq results revealed that zyxin binds to chromosomal DNA, with mitochondrial pseudogenes as the primary targets. Furthermore, we demonstrated that the LIM domains of zyxin are sufficient for DNA binding and that zyxin knockdown leads to transcriptional changes in mitochondrial pseudogenes. Additionally, zyxin knockdown impacted several other genes associated with mitochondrial integrity and apoptosis, resulting in disturbances in MTCO2P2 RNA localization, mitochondrial membrane potential, increased reactive oxygen species, alterations in the cell cycle, and progression towards apoptosis. Overall, our work shows that zyxin directly interacts with nuclear DNA and regulates the transcription of mitochondrial pseudogenes, emphasizing its role in modulating mitochondrial function.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".