Investigating the Substrate Specificity of <scp>l</scp>-Rhamnose Biosynthetic Enzymes (RmlA–D): Chemoenzymatic Synthesis of an Isosteric Nonhydrolyzable TDP-β-<scp>l</scp>-Rhamnose Phosphonate
Bibliographic record
Abstract
l -Rhamnose (Rha) is widely distributed within bacteria as a component of the polysaccharides that populate bacterial cell walls. While Rha-containing polysaccharides are highly variable in both structure and function, their presence in pathogenic bacteria has provided incentive to explore inhibition of the Rha-pathway as a means toward antibiotic development. Herein, we explore the substrate scope of the Rha biosynthetic enzymes RmlB, RmlC, and RmlD, from Aneurinibacillus thermoaerophilus, with deoxyfluoro-, aminodeoxy-, and phosphonate congeners. The isosteric phosphonate was the only congener turned over by all three enzymes demonstrating conversion to 5′-deoxythymidine- C -(1-deoxy β- l -rhamnosyl bisphosphate) (TDP-1C-Rha). Scale-up of this enzymatic process enabled the isolation of TDP-1C-Rha, an isosteric nonhydrolyzable phosphonate analogue of TDP-Rha and offers an orthogonal approach to deliver phosphonate–phosphate derived enzyme probes.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.006 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".