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Record W4412834651 · doi:10.1093/hr/uhaf198

Telomere-to-telomere genome assembly of yellow-fruited allotetraploid American ginseng ( <i>Panax quinquefolius</i> L <i>.</i> ) provides insights into flavonoid biosynthesis

2025· article· en· W4412834651 on OpenAlexaff
Xiujuan Lei, Jing Zhao, Junbo Rong, Mengyang Zhang, Jie Zhang, Xi Chen, Hui Hu, Jia Wu, Yifei Wang, Michael K. Deyholos, Liyao Su, Hui Liu, Peng Di, Jian Zhang, Ai‐Sheng Xiong, Ying‐Ping Wang

Bibliographic record

VenueHorticulture Research · 2025
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Molecular Biology Research
Canadian institutionsUniversity of British Columbia, Okanagan CampusUniversity of British ColumbiaOkanagan University College
FundersNational Key Research and Development Program of ChinaNational Natural Science Foundation of China
KeywordsBiologyGinsengGenomeFlavonoidTelomerePhytochemicalFlavonoid biosynthesisRetrotransposonGenomicsTranscriptomeBotanyTraditional medicineComputational biologyGeneticsGeneBiochemistry

Abstract

fetched live from OpenAlex

L., commonly known as American ginseng, is a valuable beneficial medicinal herb renowned for its health-promoting properties and rich phytochemical profile. Despite significant progress in understanding ginsenoside biosynthesis, the genetic basis for flavonoid diversity in American ginseng remains unclear. This study reports the first telomere-to-telomere (T2T) genome assembly for yellow-fruited American ginseng cultivar 'Zhongnongyangshen No. 2'. The genome assembly, achieved using PacBio HiFi and Oxford Nanopore Technology ultra-long read technologies, offers a high-quality reference for genomic research, addressing previous gaps in structural accuracy. Combining transcriptomic and metabolomic analyses, we investigated flavonoid biosynthesis and the regulatory mechanisms underlying fruit color variation during different developmental stages of American ginseng. Our findings highlight the phylogenetic evolution of the American ginseng genome and offer new insights into the biosynthetic pathways of anthocyanins and flavonols. This comprehensive genomic resource facilitates deeper exploration of flavonoid diversity, supports genetic improvement efforts, and enhances the potential for future applications in medicinal plant research.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.303
Teacher spread0.280 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2025
Admission routes1
Has abstractyes

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