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CRISPR/Cas9 Applications in T Cell Modifications for Tumor Immunotherapy

2025· article· en· W4414145598 on OpenAlexaff
Zhengyang Guo

Bibliographic record

VenueTheoretical and Natural Science · 2025
Typearticle
Languageen
FieldMedicine
TopicCAR-T cell therapy research
Canadian institutionsDalhousie University
Fundersnot available
KeywordsImmunotherapyT cellImmune systemCancer immunotherapyT-cell receptorCellCytokineFunction (biology)

Abstract

fetched live from OpenAlex

Cancer is a serious global health issue that has a high fatality rate. Therefore, immunotherapy as a promising tumor therapy has long-term, specific targets, et.al, benefits. The powerful gene editing tool CRISPR/Cas9 is commonly used for tumor immunotherapy in modifying immune cells such as T cells. The T cell modification research primarily focused on antigen editing, checkpoint knockout, and cytokine regulation to enhance cell targeting and immune functions. However, current studies justified the T cell immunotherapy feasibility for treating cancer, but the underlying mechanism in CAR and TCR and T cell function regulation is still not clear. This article concentrated on analyzing the CRISPR/Cas9 applications for editing T cell mechanisms for tumor cell targeting. For example, design new CARs or TCRs for either directly or indirectly bound to the tumor cell surface to target tumor cells and activate T cells. Also, analyzed tumor escape-related immune checkpoints (ICs) and the T cell function improvement method of cytokine regulations. Furthermore, this article also summarizes the drawbacks and possible improvement methods for T cell modification in tumor immunotherapy. This article provided the basic understanding and concepts of research and development for T cell immunotherapy for tumor treatment by CRISPR/Cas9 editing. However, the underlying mechanisms and more editing target genes for T cell enhancing need to be studied and discovered in further research to achieve a complete and thorough cure for cancers.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0000.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.344
Teacher spread0.333 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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