Microbial growth and activity in static incubation: spectral induced polarization (SIP) measurements of cell suspensions in non-polarizing media
Bibliographic record
Abstract
SUMMARY A growing body of literature has contributed to linking the presence of bacteria with SIP signals. Yet, there are still unresolved questions concerning the contribution of cell density and microbial metabolic activity in porous media (soils and sediments) to SIP signals. Moreover, there is continued debate on whether cells themselves polarize or whether a cell-sediment interaction is a prerequisite for the measured responses. This study investigates the SIP response of Shewanella oneidensis MR-1 in isolation, that is, in the absence of a mineral porous medium using two setups (i) cells in aqueous suspension and (ii) alginate bead-packed reactors. Results from experiments conducted with static cell suspensions shed light on the strong control of cell settling that drives erratic, poorly reproducible and difficult to interpret SIP signals. However, incubating cells in bead packed reactors yielded reproducible trends in σ″, with strong (3–10 mrad) signals that followed the expected cell growth behaviour. Relating σ″ to measured cell density and metabolic activity (using ATP) highlighted the strongly linked contribution of both activity and cell density and SIP. Here, we report a lower frequency polarization peak between 0.01 and 0.1 Hz in the bead reactors, which we attribute to the polarization of cell colonies in the densely packed reactors. In summary, our findings shed light on the direct contribution of cells and their activity to polarization, in the absence of cell-sediment interactions and provide a novel approach for studying cell polarization in static incubation in a porous environment.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".