Cross‐Tissue Specificity of Pediatric DNA Methylation Associated With Cumulative Family Stress
Bibliographic record
Abstract
Cumulative family stress (cumulative FS), characterized by mother-reported co-occurring stressors in a family context, may be biologically embedded through DNA methylation (DNAm) and contribute to later health outcomes, including psychopathology. To illuminate tissue specificity in association with cumulative FS across two accessible tissues, we compared epigenome-wide DNAm associations in buccal epithelial cells (BECs; n = 218) and peripheral blood mononuclear cells (PBMCs; n = 51) from 7- to 13-year-old children in Canada, accounting for sex, age, predicted cell-type proportion, and genetic ancestry. We found that higher levels of cumulative FS were associated with DNAm at seven sites, primarily in stress- and immune-related genes, only in PBMCs but not in BECs. Matched tissue samples (n = 50) also indicated limited cross-tissue concordance. These associations were partly explained by negative but not positive mother-child relationship. Our findings suggested that PBMC DNAm can be used as a marker for biological embedding of cumulative FS and highlighted cross-tissue specificity of the associations of DNAm with cumulative FS.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".