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Record W4416332014 · doi:10.1101/2025.11.14.688493

The BMP ligand Gdf6a Regulates Development of the Zebrafish Craniofacial Skeleton in a Pharyngeal Arch-Specific Manner

2025· preprint· W4416332014 on OpenAlexaff
Sabrina C. Fox, Andrew J. Waskiewicz, Jennifer C. Hocking

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Language
FieldBiochemistry, Genetics and Molecular Biology
TopicDevelopmental Biology and Gene Regulation
Canadian institutionsUniversity of Alberta
Fundersnot available
KeywordsZebrafishCraniofacialMorphogenesisCraniofacial abnormalityChondrogenesisSkeleton (computer programming)

Abstract

fetched live from OpenAlex

Abstract Craniofacial development involves the concerted action of several different tissue types and cellular processes that must be intricately regulated. Here, we describe the role of the BMP ligand Growth and Differentiation Factor 6a ( gdf6a ) in the development of the zebrafish craniofacial skeleton. Larval gdf6a mutant zebrafish have malformations in the midline of the craniofacial skeleton that correlate with the expression of gdf6a in the embryonic pharyngeal arches. We show that Gdf6a has arch-specific roles in craniofacial morphogenesis; Gdf6a promotes chondrogenesis and alignment of midline craniofacial elements in the posterior pharyngeal arches (arches 2 and 3) and regulates morphogenesis within the mandibular symphysis of pharyngeal arch 1. We demonstrate that Gdf6a regulates craniofacial development through activation of canonical BMP signaling, likely acting cooperatively with additional BMP ligands. Taken together, this work elucidates how Gdf6a / BMP signaling directs development of the craniofacial skeleton, specifically along the ventral midline. Summary Statement Gdf6a regulates the development of the midline structures in the zebrafish craniofacial skeleton in an arch-specific manner via canonical BMP signaling.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.360
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0010.001
Scholarly communication0.0000.000
Open science0.0010.002
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.210
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

Explore more

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