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Abstract C024: Somatic mutations in early-onset colorectal cancer: insights from a U.S. Hispanic cohort

2025· article· en· W4417209005 on OpenAlexaboutno aff
María González‐Pons, Julie Dutil, Ingrid M. Montes‐Rodríguez, Luis D. Borrero-García, Lenis Rovira-Torres, Leslie Casiano, Anna María Nápoles, Jung S. Byun, Eliseo J. Pérez‐Stable, Kevin Gardner, Marcia Cruz‐Correa

Bibliographic record

VenueClinical Cancer Research · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCancer Genomics and Diagnostics
Canadian institutionsnot available
Fundersnot available
KeywordsSomatic cellColorectal cancerGermline mutationLynch syndromeExome sequencingExomeMLH1

Abstract

fetched live from OpenAlex

Abstract Background: The alarming rising incidence of colorectal cancer (CRC) among individuals younger than 50 years (early-onset CRC) underscores the need to identify the genetic drivers to improve personalized clinical management and outcomes. The objective of this study was to characterize the somatic mutation profile of early-onset tumors from Hispanics living in Puerto Rico (HPR), a U.S. Hispanic subpopulation with a high CRC burden. Methods: Whole exome sequencing was performed using the HiSeq4000 System (Illumina) on colorectal adenocarcinoma and corresponding mucosa samples from 62 individuals with non-familial, sporadic early-onset CRC and 25 diagnosed with average-onset CRC ( >60 years old). Somatic variant calling and annotation/visualization were performed with Strelka and Ingenuity Variant Analysis software, respectively. Mutational frequency in APC, TP53, KRAS, and SMAD4 was compared to data in TCGA and AACR Project GENIE. Results: The mutational burden in early-onset CRC tumors from HPR was comparable to average-onset tumors. However, mutations in APC (p = 0.015), PIK3CA (p = 0.03), TP53BP1 (p = 0.03), and MUC16 (p = 0.02) were more common in average-onset tumors. The aflatoxin exposure signature (SBS24) was only identified in early-onset tumors, with 22.4% showing a signature fraction >10%. When compared to data on tumors in the TCGA and GENIE datasets, early-onset CRC tumors from HPR showed a distinct somatic mutational frequency in key driver genes. Conclusions: Early-onset CRC tumors display distinct somatic mutational profile compared to average-onset tumors. Additional studies with larger, diverse samples are crucial for understanding the population-specific underlying mechanisms leading to early-onset disease. Citation Format: Maria Gonzalez-Pons, Julie Dutil, Ingrid Montes-Rodríguez Montes-Rodríguez, Luis D. Borrero-Garcia, Lenis Rovira-Torres, Leslie Casiano, Anna M. Napoles, Jung S. Byun, Eliseo Perez-Stable, Kevin L. Gardner, Marcia Cruz-Correa. Somatic mutations in early-onset colorectal cancer: insights from a U.S. Hispanic cohort [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: The Rise in Early-Onset Cancers—Knowledge Gaps and Research Opportunities; 2025 Dec 10-13; Montreal, QC, Canada. Philadelphia (PA): AACR; Clin Cancer Res 2025;31(23_Suppl):Abstract nr C024.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.018
Threshold uncertainty score0.036

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.055
GPT teacher head0.440
Teacher spread0.385 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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